Dissertation > Medicine, health > Pharmacy > Pharmacology > Experimental Pharmacology

Effect of Dantrolene on the Activities of Enzymes of Glycometabolism in Rats with Myocardial Ischemia-Reperfusion

Author WangLiYan
Tutor TangYingZi
School Tianjin Medical University
Course Biochemistry and Molecular Biology
Keywords Phosphofructokinase -1 Pyruvate kinase Hexokinase Isocitrate dehydrogenase Rats Myocardial reperfusion injury Dantrolene
CLC R965
Type Master's thesis
Year 2011
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Objective: To investigate dantrolene role in ischemia-reperfusion injury in isolated rat hearts, the concept Chadan song forest dynamics of isolated rat cardiac blood flow, lactate dehydrogenase activity, infarct size, glucose metabolism enzyme activity the impact. Methods: healthy male Wistar rats (n = 48) establish Langendorff isolated heart perfusion model, divided into three groups, normal control group, ischemia-reperfusion group and dantrolene pretreatment group, the control group continued to 120 minutes of reperfusion: heart after ischemia-reperfusion group balance 30min 30 minutes of ischemia and reperfusion for 60 minutes;-by-the pretreatment group dantrolene balance after 5min amount Jiaru Dan sertraline, dantrolene perfusate at a final concentration of 5 μM continuous perfusion 30min late myocardial ischemia for 30 minutes, 60 minutes reperfusion. Each rat heart in heart rate, aortic flow, coronary flow and cardiac output was measured 5,10,20,30 min with Krebs solution. After reperfusion, TTC staining observed myocardial infarct size, the application of UV spectrophotometric determination of lactate dehydrogenase (LDH) in the perfusate after ischemia-reperfusion myocardial glucose metabolism related enzyme phosphofructokinase -1 (PFK), pyruvate kinase (PK), hexokinase (HK), and isocitrate dehydrogenase (IDH) activity. Results: 1. Dantrolene on cardiac hemodynamics and ischemia-reperfusion group compared dantrolene pretreatment group coronary flow increased (P lt; 0.01), 2 Dan Qu Lin on large LDH activity and myocardial infarct size in rat heart myocardial release dantrolene pretreatment group myocardial release of LDH activity was significantly lower than the ischemia-reperfusion group (P lt; 0.01). Compared with ischemia-reperfusion group, dantrolene pretreatment group myocardial infarct size shrink by 3. Dantrolene on rat myocardial glucose metabolism enzyme compared with ischemia-reperfusion group, dantrolene pretreatment group PFK , PK, HK and IDH activity were lower (P lt; 0.05) Conclusion: 1.5 μM dantrolene can cause the increase in coronary blood flow. Dantrolene pretreatment can reduce myocardial infarct size; reduce ischemia-reperfusion injury in myocardial cells LDH release, ischemia-reperfusion injury in myocardial protective effect. Dantrolene pretreatment group relative reduction in the activity of a key enzyme in glucose metabolism, reduce ischemia-reperfusion injury in energy demand, thus myocardial ischemia-reperfusion injury protective effect. The results of this study confirmed that dantrolene has a protective effect of myocardial ischemia-reperfusion injury. Dantrolene as myocardial protection drugs used in clinical research data.

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