The Role of Help T Cell in Oral Lichen Planus
|Keywords||Helper T lymphocytes Cytokines Chemokines Oral Lichen Planus Real-time PCR|
Lichen planus (lichen planus, LP) is a kind of skin - mucous abnormal keratinization characterized by chronic inflammatory diseases. Skin and mucous membranes can be used alone or have the disease. Occurred in the oral mucosa lesions known as oral lichen planus (orallichen planus, OLP), oral mucosal disease is the most common diseases. The etiology is unknown, chronic persistent disease, the clinical treatment has become a thorny issue, there is no cure for OLP approach. Multiple studies have shown that OLP is a T-cell mediated by the autoimmune disease, and CD4 helper T cells (T helper cell, Th cell) and cytokines in the pathogenesis of OLP and mucosal immune response plays a very important role. Current view: helper T cells can be divided into three types: type 1 (T helper cell 1, Th1), 2 type (Th2) and the recent discovery of helper T cells Th17. These three types of cells from complex cytokine and chemokine mediated regulatory networks and regulate their differentiation, and the incidence of oral lichen planus and disease development are closely related. Objective: In this study, fluorescence was detected by real-time quantitative PCR normal oral mucosa and oral lichen planus mucosal epithelium and lamina propria of connective tissue and Thl, Th2, Th17 cell differentiation and function related chemokines and cytokines: interferon-inducible protein 10 (IFN-γ-inducible protein 10, IP-10, CXCL-10), interleukin -6 (Interleukin-6, IL-6), Interleukin -17 (Interleukin-17, IL-17), Interleukin -23 (Interleukin-23, IL-23) mRNA expression investigate helper T cells Th1, Th2, Th17-type cells in the pathogenesis of oral lichen planus variation, investigate the cause of OLP for the clinical treatment of OLP offers new ideas. Method: 1. Collect clinical and pathological diagnosis of oral lichen planus mucosal tissue 10 cases and 6 cases of normal oral mucosa, the application of real-time PCR techniques were used to detect two mucosal epithelium and lamina propria of connective tissue in the IP-10, IL- 6, IL-17, IL-23mRNA expression. 2 samples were compared using the two statistical methods for IP-10, IL-6, IL-17, IL-23mRNA expression data analysis, draw conclusions. Results: 1. OLP mucosal epithelium and lamina propria of connective tissue in the IP-10mRNA expression was significantly increased compared with normal oral mucosa, a significant difference (P lt; 0.05), but OLP mucosal epithelium and lamina propria IP -10mRNA expression did not differ significantly (P gt; 0.05); 2. OLP mucosal epithelium and lamina propria of connective tissue in the expression of IL-6mRNA significantly higher than normal oral mucosa, a significant difference (P lt; 0.05 ), but OLP mucosal epithelium and lamina propria expression of IL-6mRNA no significant difference (P gt; 0.05): 3. OLP mucosal epithelium and lamina propria of connective tissue in the expression of IL-17mRNA higher than normal oral mucosa, a significant difference (P lt; 0.05), and oral lichen planus in the lamina propria IL-17mRNA expression was significantly higher than that of epithelial tissue, the difference was statistically significant (P lt; 0.05); 4. oral mucosal lichen planus epithelium and lamina propria of connective tissue in the expression of IL-23mRNA significantly higher than normal oral mucosa, a significant difference (P lt; 0.05), but OLP mucosal epithelium and lamina propria IL-23mRNA no significant difference in the expression of (P gt; 0.05). Conclusion: Compared with normal oral mucosa, oral lichen planus tissues IP-10, IL-6, IL-17, IL-23mRNA expression both in epithelial or connective tissue of the lamina propria were increased, suggesting that Thl, Th2, Thl7 type cells in oral lichen planus lesions in the development process, each play a certain role for the clinical treatment of oral lichen planus provide new ideas.