Dissertation
Dissertation > Medicine, health > Ophthalmology > Eye surgery and surgery

Morphology Study on Uveoscleral Pathway Post-Trabeculectomy with Sclera Concave Pool in Rabbit

Author PangYun
Tutor LiaoHongZuo
School Nanchang University
Course Ophthalmology
Keywords Sclera pool Trabeculectomy Uveal scleral pathway Fluorescein isothiocyanate Horseradish peroxidase
CLC R779.6
Type Master's thesis
Year 2011
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Objective: To explore the sclera pool trabeculectomy morphological changes by comparing rabbit sclera pool trabeculectomy and conventional trabeculectomy uveal scleral pathway of aqueous humor drainage may be the antihypertensive mechanism. Objects and methods: 1, the experimental objects and grouping: 30 60 adult New Zealand white rabbits, weighing 2.5-3.0Kg, according to the random number table, randomly selected 20 and 40 for the experimental group, the right eye line the sclera pool trabeculectomy The technique for the experimental group Ⅰ left eye routine trabeculectomy experimental group II. 10 of 20 blank control group did not undergo any surgery. 2 surgical methods: fornix-based conjunctival flap. The experimental group I do limbal basement, thick as the sclera 1/3 of the scleral flap, about 4mm × 4mm size, stripped to the transparent limbal within 1mm. Middle the the deep scleral under the superficial scleral flap, do the same base of 1/3 scleral thickness scleral flap (about the size of 3mm × 3mm) excised. Along the corneoscleral margin level cut 2mm × 1mm deep tissue, 10/0 line was sutured scleral flap, watertight suture conjunctival flap. The experimental group II conventional trabeculectomy operation. The postoperative observation: one week after daily slit lamp observation cornea, anterior chamber reaction, blebs, fundus. Record the proportion of the bleb, monitoring preoperative and postoperative intraocular pressure changes, and record the data for statistical analysis. UBM observation of filtration mouth outflow tract and ciliary body. Pick without complications model for further research. Fluorescent tracer experiments: one week after surgery, taking the experimental group I, experimental group II 10 each blank group 5 10 tracer 10 μl of the paracentesis injection 2mg/ml FITC-Dextran at 2 4,6,8,10 h, were sacrificed experimental group I, experimental group II each two blank group 1 and 2, the rapid removal of the eye, for frozen sections, observed under a fluorescence microscope ciliary body, the Housing pool suprachoroidal , before and after the sclera and choroidal fluorescence intensity level. 5, HRP-up experiment: one week after surgery, an alternative experimental group I, 10 in each experimental group II, 10 of the blank group 5, anterior chamber puncture injected 0.2mg/mlHRP10μ1, 60 min through carotid artery perfusion fixative 350ml , quick removal of the eye, for frozen section to observe the distribution of the flow of HRP with the aqueous humor. Parallel MASSION staining whether the observed the filtration channel proliferation of collagen fibers situations and ciliary body detachment. Results: 1 surgery results: the experimental group work and experimental group II preoperative IOP, the difference was not statistically significant (18.64 ± 2.68mmHg In 18.37 ± 2.75mmHg, the t-value of 0.32, P = 0.96), one week after the experiment group Ⅰ and experimental group II IOP compared with the preoperative significantly reduced compared two sets of IOP, the difference was not statistically significant (13.07 ± 2.66mmHg 13.31 ± 2.50mmHg t value 0.3, P = 0.29). Various parts of the fluorescence intensity fluorescent tracer results: one week after the experimental group are: most ciliary body, sclera pool choroidal fluorescence intensity before the sclera, followed by posterior scleral, choroidal fluorescence intensity minimum. Uveal scleral pathway of the experimental group Ⅰ various parts of the fluorescence intensity was stronger in the experimental group II, blank control group (P lt; 0.01). Blank control group uveal scleral way to various parts of the fluorescence intensity in the experimental group II (P lt; 0.01). 3, HRP tracking results: after 1 week experimental group I conjunctiva, the trabecular meshwork see scattered brownish yellow the HRP particle distribution, ciliary body clearance, ciliary body upper chamber, under the scleral flap, choroid, choroidal superior vena the visible dense HRP particles showed strong staining; experimental group Ⅱ conjunctiva, see intensive HRP particles showed strong staining, ciliary body clearance trabecular meshwork see the brownish yellow HRP particles scattered distribution under the scleral flap, choroid, choroidal see a small amount of HRP particles weakly staining, ciliary body and the superior vena no apparent HRP particle distribution. Under the conjunctiva of the blank control group, the trabecular meshwork, ciliary clearance, iris and ciliary body suddenly see scattered HRP particles distribution, choroid, the suprachoroidal see a small amount of HRP particles distribution. Conclusion: 1. Early the rabbit sclera pool trabeculectomy with conventional trabeculectomy antihypertensive effect is similar. Two the rabbit sclera pool trabeculectomy early postoperative aqueous humor In addition to the conventional trabeculectomy filtration extraoral drainage by sclera pool also increased uveal scleral pathway of aqueous humor drainage. With FITC-Dextran tracers and HRP tracking results Tip: the rabbit sclera pool after trabeculectomy early uveal scleral pathway of aqueous humor drainage than conventional trabeculectomy significantly increased. Uveal scleral pathway aqueous humor drainage the the rabbit sclera pool trabeculectomy early postoperative one of the primary mechanism of the ocular hypotensive

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