Dissertation
Dissertation > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor

The Expression and Significance of HMGB1、RAGE and VEGF in the Tissues of Children Wilms’ Tumor

Author ZhangGuoFeng
Tutor WangJiaXiang
School Zhengzhou University
Course Pediatric surgery
Keywords Child Wilms tumor High mobility group protein-1 Advanced glycation end products receptor Vascular endothelial growth factor Immunohistochemistry
CLC R737.11
Type Master's thesis
Year 2011
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The background and purpose of Wilms' tumor, also known as Wilms' tumor (Wilms' tumor), infants and young children the most common abdominal malignant solid tumors, also known as the embryonic kidney tumor. Is the most common pediatric urinary tract cancer, accounting for 80% of urinary tract tumors in children under the age of 15, accounting for 8% of the abdominal solid tumors in children. In recent years after surgery, chemotherapy, radiotherapy treatment, the prognosis of Wilms' tumor in children with malignant solid tumors in the most ideal, Wilms tumor significantly improved long-term survival. But there are still some patients because of tumor recurrence leading to death, therefore, the analysis of Wilms tumor invasion and metastasis mechanism, able to make an early diagnosis and early relapse of Wilms' tumor found in Wilms tumor prognosis has significance. Inflammatory mediators high mobility group protein -1 (HMGB1) in recent years with the body of advanced glycation end product receptor (RAGE) role in tumor invasion and metastasis has attracted everyone's attention, reported in the literature with oral cancer, gastric cancer, breast cancer, cervical cancer, lung cancer, colorectal cancer, pancreatic cancer and other tumor growth, invasion and metastasis. Vascular endothelial growth factor (VEGF) is currently considered the main angiogenic growth factor to stimulate angiogenesis in the tumor tissue, plays an important role in the tumor vascular invasion and metastasis. Explore HMGB1, RAGE and VEGF expression in children with Wilms' tumor tissue in tumor vascular invasion, lymph node metastasis of Wilms tumor prognosis is of great significance. Methods First Affiliated Hospital of Zhengzhou University, January 2003 to January 2010, pediatric surgical resection with pathologically confirmed Wilms tumor paraffin-embedded specimens of 50 cases, the same period in the corresponding tumor adjacent tissues of 15 cases and 7 cases of kidney disease deaths The autopsy of children with normal kidney tissue samples. Application SP immunohistochemical staining using SPSS 13.0 software for data analysis, χ2 test and Fisher's exact test was used to compare the expression positive rate, econometric analysis of tumor expression using the average integrated optical density (AIOD), supplemented by computer image analysis system analysis methods, data are expressed as mean ± standard deviation, Student t test was used to compare the group differences between using pearson correlation analysis, the Kaplan-Meier method survival analysis was performed by the Long-rank test, test level a = 0.05. Results 1 HMGB1 expression in the tumor group, adjacent to the tumor and normal kidney tissue group In this study, 50 cases of Wilms' tumor specimens of HMGB1 in tumor group, adjacent to the tumor group and normal kidney tissue group expressed positive rates were : 72%, 33.3%, and 14.2%, respectively. The tumor group peritumoral group of normal kidney tissue group compared the differences were statistically significant (P lt; 0.05), adjacent to the tumor group and normal kidney tissue groups showed no significant differences (P gt; 0.05). HMGB1 in clinical stage Ⅲ ~ Ⅳ stage and poor prognosis tissue-type group and expression in Wilms tumor tissue of lymph node metastasis group (148.69 ± 12.17,157.88 ± 4.44,152.11 ± 7.36) was significantly higher than the clinical stage Ⅰ ~ Phase II and the prognosis is good tissue-type and without lymph node metastasis group (139.23 ± 5.83,139.52 ± 5.22,138.61 ± 5.59) (P lt; 0.05). HMGB1 expression in Wilms tumor tissue and patient gender and tumor size (P gt; 0.05). HMGB1 positive expression of children's survival rate was significantly lower than children with negative expression (P lt; 0.05). In this study, 50 cases of Wilms' tumor specimens of 2 RAGE expression in the tumor group, adjacent to the tumor group and normal kidney tissue group, the positive rate of RAGE expression in the tumor group, adjacent to the tumor and normal kidney tissue group were: 84%, 53.3%, O. The tumor group peritumoral group, normal kidney tissue group compared the differences were statistically significant (P lt; 0.05), a significant difference (P lt; 0.05) adjacent to the tumor group compared with normal kidney tissue group. RAGE expression in clinical stage Ⅲ ~ Ⅳ stage and poor prognosis tissue-type group and lymph node metastasis group (147.73 ± 6.71,155.29 ± 3.97,151.56 ± 5.46) was significantly higher than the clinical stage Ⅰ ~ Ⅱ stage and the prognosis is good organization type without lymph node metastasis group (133.30 ± 11.23,135.39 ± 9.71,133.00 ± 8.75) (P lt; 0.05). Independent of RAGE expression in Wilms tumor tissue of patients' gender and tumor size (P gt; 0.05). The survival rate of children with positive expression of RAGE significantly lower than children with negative expression (p lt; 0.05). 3 VEGF expression in the tumor group, adjacent to the tumor group and normal kidney tissue group in this study, 50 cases of Wilms' tumor specimens, the rate of positive expression of VEGF in the tumor group, adjacent to the tumor and normal kidney tissue group were: 76%, 40% and 14.2%, respectively. Next to the tumor group with tumor group compared to normal kidney tissue group differences were statistically significant (P lt; 0.05), compared with normal kidney tissue group adjacent to the tumor group, no significant difference (P gt; 0.05), VEGF in clinical staging Ⅲ - Ⅳ period and a poor prognosis tissue type group and lymph node metastasis of Wilms' tumor tissue expression (163.14 ± 7.50,169.17 ± 4.42,163.83 ± 7.20) was significantly higher than the clinical stage Ⅰ - Ⅱ stage and prognosis well-organized and without lymph node metastasis group (140.85 ± 8.87,143.94 ± 10.39,141.18 ± 8.95) (P lt; 0.05). Independent of VEGF expression in Wilms tumor tissue of patients' gender and tumor size (P gt; 0.05). VEGF expression in children's survival rate was significantly lower than children with negative expression (P lt; 0.05). 4 of HMGB1, HMGB1 and RAGE expression of RAGE and VEGF in Wilms tumor tissue expression analysis in this study, 50 cases of Wilms' tumor tissue there is a positive correlation (r = 0.424, P = 0.002); HMGB1 and VEGF expression was positively correlated (r = 0.453, P = 0.001); RAGE and VEGF expression no reflected (r = 0.237, P = 0.101). Conclusion of HMGB1, RAGE and VEGF three showed high expression in Wilms tumor tissue, their high expression affect prognosis of Wilms' tumor. 2 HMGB1, RAGE and VEGF three Wilms tumor with different clinical stages, a significant difference in expression of different histopathological types, suggesting that they play an important role in the occurrence of Wilms tumor development. 3 HMGB1, RAGE and VEGF three Wilms tumor expression in lymph node metastasis was significantly higher than that without lymph node metastasis, and HMGB1 to RAGE and HMGB1 expression of VEGF was positively correlated, indicating that they are in the lymph nodes of the Wilms' tumor collaborative play an important role in the transfer. 4 HMGB1, RAGE and VEGF three Wilms tumor lymph node metastasis, plays an important role in angiogenesis joint detection Wilms tumor tissue HMGB1, RAGE and VEGF expression, help predict Wilms tumor prognosis of children.

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