Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology

The Influence of Aconitum Carmichaeli on Ashenia Cold Syndrome Rats and Anemarrhenae Rhizoma on Asthenia Hot Syndrome Rats on Whole Genome Expression of Liver

Author HanBingBing
Tutor WangShiJun
School Shandong University of Traditional Chinese Medicine
Course Chinese and Western medicine combined with the basis
Keywords Aconitum Carmichaeli Asthenia cold syndrome Anemarrhenae Rhizoma Asthenia hot syndrome Gene chip
CLC R285
Type PhD thesis
Year 2011
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Objective: To discuss asthenia cold syndrome rat models and asthenia hot syndrome rat models and the evaluation system to superficial characteristics fit in with throry of Traditional Chinese Medicine, the Asthenia cold syndrome rat models and asthenia hot syndrome rat models were induced by compound preparation of traditional chinese medicine, which were estimated by PLS data analysis. To analyse the molecular mechanism and the related mechanism of property and effect of Chinese medicinal herbs with hot and cold property from the view of gene expression spectrum,we study the influence of Chinese medicinal herbs with hot property such as Aconitum carmichaeli on asthenia cold syndrome rats, herbs with cold property such as Anemarrhenae Rhizoma on asthenia hot syndrome rats on whole genome expression of liver by gene chip.Methods: The rat models were induced by compound preparation of traditional chinese medicine. Asthenia cold syndrome rats were induced by compound preparation of Raw Gypsum, Radix gentianae, Cortex Phellodendri, Anemarrhenae Rhizoma and asthenia hot syndrome rats were induced by compound preparation of Aconitum carmichaeli,Cinnamomi Cortex,Rhizoma Zingiberis once daily for 14 days. After the models were copied ,the model rats were estimated by PLS data analysis and divided into groups according to the results of analysis. Then the asthenia cold treatment group was treated by Aconitum carmichaeli,and asthenia hot treatment group was treated by Anemarrhenae Rhizoma once daily for 7 days. Seven days later,the rat liver gene expressions in each group were detected by RatRef-12 gene array made by Illumina Company. We selected the differential expression genes , analyzed the genes by cluster and conducted the significant analysis on the genetic function of differential genes. A part of genes were selected to test the accuracy of results by PCR. Results: PLS analysis shown that the sample points of syndrome of asthenia cold syndrome group, asthenia hot syndrome group and control group was completely separated, which was clearly differentiated. The important contribution indicators to syndrome of asthenia cold model group and blank control model group were such as stool texture, hair cleanliness, urine color, weight, average toe temperature, spontaneous activity, water intake, urine texture. The important contribution indicators to syndrome of asthenia hot model group and blank control model group were such as stool texture, stool color, average toe temperature, body weight, rectal temperature, metabolic energy to unit weight, activity. There were 99 strips of differential expression gene, and 8 items of significant gene function such as immune response, defense response, response to other organism in asthenia cold model group as compared to the control group. There were 212 strips of differential expression gene, and 31 items of significant gene function such as immune response, defense response, inflammator response, oxidoreductase activity, chemokine receptor binding,chemokine activity in the asthenia cold treatment group as compared to the asthenia cold model group. There were 99 strips of differential expression gene, and 5 items of significant gene function such as defense response , sterol metabolic process in the asthenia hot model group as compared to the control group. There were 67 strips of differential expression gene and 5 items of significant gene function such as defense response, inflammatory response, chemokine receptor binding,chemokine activity in the asthenia hot treatment group as compared to the asthenia hot model group. There were 114 strips of differential expression gene, and 4 items of significant gene function such as organic and carboxylic acid biosynthetic process in the asthenia cold model group as compared to the asthenia hot model group. There were 28 strips of differential expression gene, among which 16 stips were about immune response among asthenia cold model group,asthenia hot model group and control group,.Conclusion: The main symptoms and signs of asthenia cold syndrome and asthenia hot syndrome model animal were consistent with the clinical diagnosis of asthenia cold syndrome and asthenia hot syndrome, which reflected the pathological features of“deficient cold endogenous heat”of asthenia cold syndrome and“yin deficiency endogenous heat”of asthenia hot syndrome.Many strips of gene about response to stimulus were down-regulated in asthenia cold syndrome rats,which induced down regulation of function about immune response, defense response, response to other organism. The molecular mechanism of“insufficiency of yang qi”of asthenia cold syndrome was possibly related to these genes. The regulation of Aconitum carmichaeli on genes about immune response and oxidoreductase activity was possibly related to the molecular mechanism of“warming yang and dissipating cold”. The abnormal expression of genes about defense response and sterol metabolic process in asthenia hot syndrome rats was possibly related to the molecular mechanism of asthenia hot syndrome. The regulation of Anemarrhenae Rhizoma on genes about response to stimulus was possibly related to the molecular mechanism of“enrich yin”.The genes about immune response diffrently changed both in asthenia cold model group and asthenia hot model group,which was possibly related to the molecular mechanism of“lack of vital essence resulting in deficiency syndrome”. The abnormal expression of genes about oxidoreductase activity was possibly related to the different molecular mechanism of asthenia cold model group and asthenia hot model group.

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