Effect of the Compound Rhizoma Curcumae Powder on Expression of VEGF and RANTES in Endometrium of Patients with Endometriosis and Clinical Efficacy Observation
|School||Tianjin Medical University|
|Course||Traditional Chinese Medicine|
|Keywords||Compound Curcuma scattered Endometriosis Clinical Observation Angiogenesis Inflammatory immune response VEGF RANTES|
Compound the Curcuma scattered supervisor Professor Cao Baoli developed based on the mechanism of endometriosis (endometriosis) blood stasis, and years of clinical experience of treatment of endometriosis drug granules. The preparatory work for the preparation of internal the endometriosis rats pharmacodynamic test, this study further the herbal compound Ezhu bulk of clinical efficacy comparative observation and clinical studies to explore its mechanism. Objective 1 observed Compound Ezhu by scattered Dafei Lin used alone or in conjunction with in moderate to severe endometriosis after laparoscopic surgery drugs consolidating role in the treatment and to explore the clinical application value the compound Ezhu scattered. Explore the expression changes and significance in patients with ectopic and eutopic endometrium of endometriosis, including vascular endothelial growth factor (VEGF) and the chemokine RANTES. 3 to study the the bulk Compound Ezhu by internal endometriosis patients with ectopic and eutopic endometrial VEGF and I (?) ANTES expression, so as to explore the mechanism of action of compound Curcuma scattered to find a theoretical basis for further clinical application. Method 1, retrospective analysis from June 2007 to June 2009 in our hospital gynecological laparoscopic preserve fertility in surgical treatment of 115 patients with moderate to severe endometriosis clinical data is divided into the Curcuma scattered group (53 postoperative treatment programs cases), Dafei Lin group (36 cases), Dafei Lin 10th Curcuma scattered group (26 cases). Curcuma scattered groups in one week after the start given compound Curcuma scattered (drugs: Curcuma 15g Trigonobalanus 15g, the epimedium 15g, Astragalus 15g, Yuan Hu 10g, respectively granules made of by Jiang Yin Tianjiang Pharmaceutical Co., Ltd.) , 2 times a day, and even served 3-6 months. Dafei Lin group postoperative the 7d intramuscular Dafei Lin 3.75mg, once every four weeks, three times in a row. Dafei Lin Curcuma scattered group dosing regimen with two single-drug group. After 3,6,12 observe the clinical efficacy of the patients in each group (including mitigation, improved relapse), pain scores, incidence of adverse reactions ≥ 18 months. 2, were detected by immunohistochemical SP method to our hospital from July 2009 to January 2011 the gynecological due to the ovarian endometriosis laparoscopic surgical treatment of 23 patients with preoperative Compound the Curcuma San 3 months of treatment (regimens with ectopic and eutopic endometrium of the clinical observation Curcuma the scattered group) of patients with endometriosis (treatment group), 30 patients with untreated patients with endometriosis (endometriosis) and 30 cases of uterine fibroids patients (control group) endometrial expression levels of VEGF and RANTES. Results 1 Clinical Observation: Curcuma scattered group recurrence rate was 1.89%, and 2.78% Dafei Lin, Dafei Lin Curcuma scattered group without recurrence; clinical efficacy of three treatment regimens and pain score differences were not statistically significant (both P gt; 0.05). The incidence of adverse reactions Curcuma scattered group (11.32%) than Dafei Lin group (86.11%) and Dafei Lin Curcuma scattered group (88.46%) was significantly lower (all P lt; 0.01). 2, patients with endometriosis ectopic reign the intima of VEGF and RANTES expression by immunohistochemical staining observed by light microscopy of VEGF and RANTES are mainly expressed in gland epithelial cells, part of the interstitial cytoplasm seen scattered in the expression. The endometriosis patients ectopic expression of VEGF and RANTES protein can be detected eutopic endometrial endometrium and control group. The endometriosis eutopic endometrium of VEGF and RANTES expression levels were significantly higher than the control endometrium (all P lt; 0.05), the level of VEGF expression in ectopic endometrium was significantly lower than the reign of endometrial, and higher than the endometrium, the differences were statistically significant (P lt; 0.05). RANTES expression levels were significantly higher in the ectopic endometrial eutopic endometrium (P lt; 0.001), and the difference was statistically significant. VEGF and RANTES in the ectopic expression levels between the respective proliferative phase and secretory phase in eutopic endometrium and endometrial control group comparison, the differences were not statistically significant (P gt; 0.05). Reign the intima of VEGF and RANTES expression levels were significantly higher than in the proliferative and secretory midterm intima of the corresponding period of the control group, the difference was statistically significant (P lt; 0.05). Ectopic and eutopic endometrium of endometriosis patients, VEGF, and RANTES compared the expression levels of the different clinical stages were not significantly different sex (P gt; 0.05). Do in the same period of moderate to severe endometriosis, RANTES expression in ectopic endometrium was significantly higher than in eutopic endometrium, the difference was statistically significant (P lt; 0.05). 3 Compound Ezhu by scattered Endometriosis patients ectopic reign of the intima of VEGF and RANTES expression by immunohistochemical staining observed by light microscopy, I Compound Ezhu by scattered after 3 months of treatment, the expression of VEGF and RANTES positive cells than The endometriosis reducing positive brownish yellow protein relief. Ectopic endometrium of the treatment group, the reign of the intima of VEGF and RANTES expression levels were significantly reduced compared with endometriosis, the difference was statistically significant (all P lt; 0.05). Conclusion 1, Compound Ezhu by scattered in the treatment of moderate to severe endometriosis after laparoscopic surgery drugs consolidate disease play a better role in the control, you can reduce the relapse rate, and adverse reactions with the value of clinical application. 2, patients with endometriosis ectopic and eutopic endometrium of VEGF and RANTES expression was significantly enhanced development is closely related to the occurrence of endometriosis. High expression of the reign of the the intima VEGF and RANTES is expected to become the new ways of early diagnosis of endometriosis and treatment. 3, compound Curcuma scattered able to cut patients with endometriosis ectopic reign the intima of VEGF and RANTES expression levels, thus pre eutopic endometrial biological characteristics in the inhibition of endometrial tissue neovascularization and inflammatory immune response play a therapeutic role.