Experimental Study on the Pharmacological Mechanisms on Cellular Immunity of BiQingYin for the Treatment of Rheumatoid Arthritis

Objective: To study in rheumatoid arthritis (RA) pathogenesis explore clean drinking anti- RA paralysis cellular immune regulatory mechanism in cellular and molecular level, as Chinese medicine and clinical application of anti- RA further pharmacological studies provide a scientific basis . Methods: Jurkat cells as a model to TGP (TGP), dexamethasone (DEX), methotrexate (MTX) for the control drug , using flow cytometry , MTT, ELISA immunological methods to observe the paralysis clean drinking of Jurkat cell proliferation , differentiation, cycle ; peripheral blood of patients with RA activation of T lymphocytes . Results: Bi clean drinking inhibits proliferation of T lymphocytes , CD69, CD25 expression , decreased production of IL-2 and IFN-γ expression in the model group were significantly different (p lt; 0.01 or p lt; 0.05 ) , and TGP \\ DEX \\ MTX group were no significant differences (p gt; 0.05). Clean drinking can cause paralysis Jurkat cells Gl arrest , Gl phase cell accumulation, can not enter S phase arrest Gl to S phase transformation process , so that the G2 / M phase cells are relatively increased. Conclusion: Bi clean drinking on RA immune regulatory mechanisms may be: by inhibiting the activation of T cells , reduced IL-2 and IFN-γ production indirectly inhibit Th differentiation and proliferation , thereby reducing joint and / or synovial inflammation and hyperplasia , slow down the process of bone destruction ; T cells by blocking the synthesis of energy and raw materials , reduce cell G2 phase transformation to promote the abnormal proliferation of synovial cell apoptosis.