Studies on Molecular Genetics of Japanese Scallop (Patinopecten Yessoensis)
|School||Ocean University of China|
|Keywords||Yessoensis C. farreri Chlamys nobilis AFLP Microsatellite Mitochondrial genome Genetic Diversity Linkage map Pedigree analysis Mode of inheritance Phylogeny|
1. Yessoensis cultured population genetic diversity study yessoensis introduced more than 20 years of history in China. Cultured populations reduce genetic diversity has become a major area of ??concern. In this study, using six microsatellite loci, genetic diversity analysis of three breeding populations and two wild populations. Studies have shown that the breeding population genetic diversity was significantly lower than the wild population. Farmed and wild populations of alleles average of 5.7 and 7.9, respectively, average expected heterozygosity were 0.582 and 0.671. Reduced genetic diversity due to the nearly 20 years of isolation and genetic drift. F st index shows that five groups in the presence of significant genetic differences. Compare the paired allele distribution among populations, also found that there are genetic differences between the groups. Clustering tree based on genetic distance drawn Neighor-joining farmed and wild populations will be clearly classified into two categories. Farmed and wild populations of this study, the genetic diversity of information provides important data for yessoensis germplasm conservation and genetic improvement of selective breeding. 2. Yessoensis genetic linkage map constructed using AFLP and SSR marker technology combined testcross strategy initially built yessoensis, male and female genetic linkage map. 32 pairs of AFLP primer combinations 413 1:1 separation mark (209 from the female parent, 204 from the male parent). 6 microsatellite loci, four parental polymorphic. Two sites as the female parent separation mark, the other two sites for the two pro-separation of the total marks. Female linkage map included 20 linkage groups, the total length of the map for 2198.8cM; the male map consists of 21 linkage groups, the total length of the map for 2137.6cM. More uniform distribution of AFLP markers in the map, the average marker interval of 14.7-15.6cM. Map coverage of male and female parents are 77.9%. This study is the first to build a genetic map of the yessoensis, its genome research laid the foundation, and also provides the foundation for the establishment of the high-density mapping yessoensis framework. 3. The mode of inheritance of microsatellite markers in its pedigree identification application of this research yessoensis 4 control mating pedigrees to detect seven microsatellite markers genetic pedigree larvae mode, at the same time to explore these markers in yessoensis The pedigree Identification feasibility. The study showed that the seven microsatellite markers are in line with Mendelian genetics. No sites sex genetic selective fertilization between gametes of each parent there is no obvious selectivity. Two sites in two pedigrees null allele display microsatellite markers for population genetic analysis, genetic mode of research is very necessary. While calculating the genetic distance null allele as homozygous offspring from four families in the neighbor-joining software is still accurate identification. This result proves that these microsatellite markers can be used effectively the scallop larvae paternity test, and hence can be applied to investigate the effective number of parents yessoensis cultured population. 4. Ezo scallop mitochondrial genetic model so far, bivalve mitochondrial genetic model studies focused on the mussels. In this study, by 10 yessoensis larvae pedigree application DGGE method the mitochondrial for some noncoding region (NcR2), genetic patterns are discussed. The results show that all pedigree the larval offspring only detected the female parent haplotype yessoensis mitochondrial maternally inherited. 5. Metazoan mitochondrial fan Beko molecular phylogeny based on mitochondrial DNA sequence typically includes 37 genes. These genes contain important information system evolution. Chlamys farreri and the Chlamys nobilis this study were measured mitochondrial genome sequence and analyzed its gene composition and genomic arrangement to measure the meristematic value in the analysis of two sets of genome sequences in the system. The the mitochondrial total length of Chlamys farreri 21695bp, Chlamys nobilis 7693bp. Chlamys farreri, Chlamys nobilis contains 12 protein-coding genes, lack Atp8 gene. These characteristics and the same has been reported in other bivalve mitochondrial. Sequence in the presence of a long non-coding region of C. farreri. In GenBank Ezo scallop and the bay scallop mitochondrial genome sequence based on our analysis of the the four scallops protein gene order. The results show that the order of the four the scallops protein coding genes are very similar. Neighbor-joining cluster analysis tree through the analysis of protein-coding gene sequences, four scallops, results show that the closer relationship Chlamys nobilis and yessoensis.