Clinical Research of Serum Survivin in Patients with Rheumatoid Arthritis
|School||Tianjin Medical University|
|Keywords||Arthritis Rheumatoid Bone erosion Survivin Enzyme-linked immunosorbent assay|
Objective: To detect rheumatoid arthritis (Rheumatoid Arthritis, RA) and joint damage in patients with serum different hierarchical levels of survivin and its clinical and laboratory parameters such as correlation analysis, preliminary study in RA patients and serum levels of survivin in RA joints undermine the role of the occurrence and development. Methods: 64 cases of clinically diagnosed RA patients and 20 healthy serum. According to the RA RA clinical remission criteria active RA patients were divided into groups, remission RA group, according to the ACR diagnostic criteria for grading and joint damage in RA patients by staging joint X-ray bone erosion will be divided into groups of RA patients, non-bone erosion groups. Double antibody sandwich enzyme-linked immunosorbent assay (ELISA) Detect 64 RA patients and healthy control group, serum survivin levels while collecting patients with rheumatoid factor (RF), Hang-cyclic citrullinated peptide (CCP) antibodies, antinuclear Zhou factor (AKA), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and immunoglobulin (IgG, IgA, IgM) and blood count and record the number of swollen joints in patients using (28-joint count), tenderness joint count (using 28 joint count), duration of morning stiffness (min), disease activity index 28 and staging joint X-ray data and application SPSS17.0 statistical software for statistical analysis, for each set of data comparing differences and correlation analysis. Results: 1.RA serum survivin levels (307.54 ± 137.43pg/mL) was significantly higher than the normal control group (105.22 ± 34.86 pg / mL), χ2 = 21.69, P lt; 0.01, the difference was significant statistical significance. 2 active RA serum survivin levels (324.72 ± 141.59 pg / mL) and remission RA group (265.92 ± 113.81 pg / mL) compared to, X2 = 0.59, P gt; 0.05, the difference was not statistically significant. 3.RA combined bone erosion serum survivin levels (380.68 ± 97.21 pg / mL) than RA bone erosion in unconsolidated group (161.26 ± 73.96 pg / mL), χ2 = 15.68, P lt; 0.01, the difference was statistically significant significance. 4.RA unconsolidated bone erosion serum survivin levels (161.26 ± 73.96 pg / mL) and normal control group (105.22 ± 34.86 pg / mL) compared, X2 = 1.91, P gt; 0.05, the difference was not statistically significant. 5.RA serum survivin levels and duration, CRP, anti-CCP and RF factor was positively correlated (correlation coefficient r, respectively: 0.453,0.394,0.264,0.253, the corresponding P values ??were 0.000 lt; 0.01,0.001 lt; 0.01 , 0.037 lt; 0.05,0.046 lt; 0.05), with swollen joint count, tender joint count, hemoglobin, platelets, IgG.IgA so there was no correlation (P gt; 0.05). 6.RA group of patients with bone erosion of longer duration (P lt; 0.01); anti-CCP antibodies, RF.ESR and CRP were significantly higher than in the group of non-bone erosion bone erosion group, the differences were statistically significant (P lt ; 0.05); AKA the positive rate of bone erosion group compared with non-bone erosion is also higher (P lt; 0.05); 7.RA patients with bone erosion survivin levels and serum anti-CCP antibodies, CRP and WBC relevant sex (t values ??were 7.544,2.041,2.145, corresponding both P lt; 0.01). 8. Survivin joint destruction in RA could predict, combined with anti-CCP antibodies predict a greater role. survivin positive predictive value of 0.69, anti-CCP antibodies, RF, AKA, CRP and survivin CCP antibody positive predictive values ??were 0.72,0.51,0.71,0.71 and 0.74 Conclusion: 1.RA serum survivin levels were significantly higher than normal control group, suggesting that survivin in the pathogenesis of RA may have some significance. 2 active and remission RA patients serum levels of survivin little difference was not statistically significant, and with tender joint count, swollen joint count, erythrocyte sedimentation rate and DAS28 score and other non-correlated, suggesting that survivin may be associated with RA activity unrelated. 3.RA survivin combined serum levels of bone erosion than RA group unconsolidated bone erosion and bone erosions in patients with RA unconsolidated serum levels of survivin little difference with the normal control group; while RA patients with bone erosion survivin levels of serum anti-CCP antibodies, CRP and WBC were correlated, suggesting that survivin may be involved in the pathogenesis of RA bone destruction and disease severity. 4.Survivin joint destruction in RA could predict, combined with anti-CCP antibodies predict a greater role.