Dissertation
Dissertation > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Other

The Correlation of T Lymphocyte Subsets and Serum Endogenous Cortisol in Peripheral Blood in COPD and Asthma

Author LiYanBing
Tutor LiuQianZhong
School Nanchang University
Course Internal Medicine
Keywords Chronic obstructive pulmonary disease (COPD) Asthma T cell subsets Endogenous cortisol Cellular immunity Quantitative flow cytometry (QFCM) Radioimmunoassay (RIA)
CLC R563.9
Type Master's thesis
Year 2011
Downloads 27
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Objective: To observe the chronic obstructive pulmonary disease (COPD) and asthma patients peripheral blood T cell subsets and serum endogenous cortisol, to provide a reference for COPD and asthma, early intervention, treatment and prognosis. Methods: 40 cases of patients with COPD, including 24 males and 16 females, mean age 67.53 ± 6.92 years, according to the disease is divided into mild group (15 cases), 25 cases of severe group. 30 cases of patients with asthma, including 11 males and 19 females, mean age 40.97 ± 15.96 years old, divided into a control group of 12 patients according to the symptoms of asthma control, not the control group, 18 patients. Control group of 30 patients were healthy subjects, including 18 males and 12 females, mean age 63.57 ± 8.12 years. All objects nearly a month without oral or intravenous glucocorticoids and immunosuppressive agents. Peripheral venous blood collected at eight o'clock in the morning, quantitative flow cytometry assay peripheral blood CD3, CD4, CD8 T cells, the absolute number of serum cortisol radioimmunoassay. Clinical data compared using one-way ANOVA, pairwise comparisons homogeneity of variance using the of q test or unequal variances Games-howell inspection, the groups were compared using independent sample t test, and related information for Pearson bivariate correlation analysis. Results: 1 with COPD group CD3 T cell count lower than asthma group (p lt; 0.05), asthma group than in the control group, but no significant differences (p gt; 0.05); CD4, and CD8 T cell counts in the COPD group than in the asthma group and reduce the control group (p lt; 0.05), asthma group than in the control group, but no significant differences (p gt; 0.05). Severe group of COPD and asthma uncontrolled group of CD3, CD4, CD8 T cells with the control group, a statistically significant difference (p lt; 0.05). 2 serum endogenous cortisol control group 8.47 ± 3.02μg/dl The COPD group was 3.61 ± 1.99μg/dl asthma 5.88 ± 3.16μg/dl. Serum cortisol in COPD, asthma group than the control group (p lt; 0.01), and the COPD group than in the asthma group (p lt; 0.05). COPD with mild, severe group, uncontrolled asthma group serum cortisol control group statistically significant difference (p lt; 0.05), while no significant difference in asthma control group and the control group. COPD severe group age is higher than the mild group (t = -4.483, p lt; 0.01), disease duration longer than the mild group (t = -2.964, p lt; 0.01), COPD severe group CD4 T cells, serum corticosterone low alcohol less severe disease group (t = 3.694,3.347, p lt; 0.01), but CD3, CD8 T cells was no significant difference (t = 1.789, -0.139, p gt; 0.05). Uncontrolled asthma and control groups, CD3, CD4, CD8 T cell counts increased (t = -4.363, -4.481, -2.986, p lt; 0.01), and reduce serum cortisol (t = 3.955, p lt ; 0.01). 5. COPD and asthma in both groups aged CD3, CD4, CD8 T cells, serum cortisol was negatively correlated (r = -0.405, -0.461, -0.264, -0.454, p lt; 0.05), the course of the two groups There was a negative correlation (r = -0.328, -0.326, -0.203, -0.376, p lt; 0.01), the two groups of subjects serum cortisol CD3, CD4, CD8 and CD3, CD4, CD8 T cells, serum cortisol T cells were positively correlated (r = 0.573,0.630,0.397, p lt; 0.01). Conclusion: T-cell subsets are involved in COPD and chronic airway inflammation in asthma blog are the important cells, the longer the duration, the more severe the disease, the cellular immune changes may be more obvious; Serum cortisol affect the patient's condition changes with age extension of the course and dropped, and the more obvious changes in disease onset or worsening. Cellular immune disorders and adrenal function decline in mutual contact may play a synergistic role in the disease development process.

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