The Mechanism of the Effect of DM and Age Increasing on Erectile Dysfunction
|Keywords||Erectile dysfunction Increasing age Diabetes Synergy Connexin|
The dissatisfaction Background: With the increase in life expectancy raise the level of diabetes treatment and diabetes patients, people gradually recognize that diabetes mellitus (DM) erectile dysfunction (ED) has seriously affected the quality of life, sex life, sexual dysfunction become an important issue affecting the patient's quality of life, and even corrected age, gender, and other variables that affect the quality of life issues still have a significant impact on the quality of life. The diagnosis and treatment of ED has attracted the attention of clinical experts ED treatment can improve the patient's quality of life. But the study of mechanisms of diabetic ED is lagging behind, to affect DM of ED treatment effect. At present, the increasing age for male erectile dysfunction has been clinically proven. A common complication of diabetes, erectile dysfunction has been clinically proven. However, the mechanism of increasing age and diabetic penis erectile dysfunction is still very difficult to clear, increasing age of the synergistic effects of erectile dysfunction with diabetes, we still have further study. The gap junctions (gap junction), also known as gap junction is formed by connecting a special film structure adjacent to the connecting passage between the two cells arrayed. Electrical coupling adjacent cells through gap junction-mediated gap junctional intercellular communication (gap junction intercellular communication, GJIC) the exchange of information, energy and matter, to participate in the exchange of substances between the cell metabolic coupling and electrical signals pass , cell metabolism, homeostasis, plays an important role in the regulation of physiological processes such as proliferation and differentiation. The main component of the corpus cavernosum smooth muscle cell gap junction connexin 43 (of CX43 in) of CX43 in corpus cavernosum smooth muscle cells by electrical coupling and metabolic coupling links into a functional whole, can coordination between many smooth muscle cells synchronization diastolic important role on the the induced corpora cavernosa smooth muscle relaxation and maintain an erection. Through research with aging and diabetes on erectile function in rats, investigate the pathogenesis of DM and Ageing ED to provide a theoretical basis for the treatment of ED of DM and aging. The first part of the Age of erectile function mechanism Objective: To establish the rats during the aging model, and to observe the impact of aging on erectile function in rats. Methods: 1,3-month-old, 9-month-old, 18-month-old experimental animals, n = 10. In one week after the feeding, each rat was observed after injection of apomorphine (APO, 80μg/kg) penis of rats with or without an erection, penile erections. Glans hyperemia and the end of the penis body for erectile. 2, take 3 months old, and 9-month-old, 18-month-old rats, the base of the penis albuginea cavernous tissue, ultrathin sections (70nm thick), citrate stained with lead in the TEM-1 200EX transmission electron microscope observation . Application Realtime PCR, Western blot detection of aged rats with the younger rat corpus cavernosum gap connexin 43 (CX43) expression results: 1 aged rats erections and erectile rate decreased significantly compared to the young rats, the two group, the difference was statistically significant (erections) or highly significant (the erection rate) (Table 1). Age 2, along with rats endothelial cells and smooth muscle cells in cell disorder, separation, increased interstitial tissue between them, the uneven distribution of the increased number of mitochondria, but structural disorder and mitochondria reduce the swelling of mitochondria, vacuoles increased changes . Real-time PCR detection CX43 gene expression results: with increasing age, the rat corpus cavernosum CX43 expression gradually decreased, reveal senile rat erectile dysfunction rat corpus cavernosum CX43 decreased expression of closely related . Western blot test results show that, CX43 protein expression age increase gradually reduce. Conclusion: 1, aged rat erectile rates, the number of penile erection was significantly lower than the younger rats, significantly reduced erectile function with age. 2, the aged rats stem cavernous tissue of CX43 expression was significantly lower than young rats reveal the age rats erectile dysfunction with rat corpus cavernosum CX43 decreased expression is closely related. Mechanism of erectile function in the second part of the diabetic rats Objective: To study the mechanism of diabetes erectile dysfunction. Method: 1 DM animal models. The experimental animals were divided into experimental group of 40 (including diabetic model group (n = 20), 20 model treatment group), control group 20. 12 weeks after injection of apomorphine (APO, 80μg/kg) observed three groups of rats with or without an erection of the penis, penis erections. Glans hyperemia and the end of the penis body for erectile. 2, the experimental rats were sacrificed in the 12th week, dissecting the penile tissue. The cavernous tissue ultrathin sections (70nm thick) Take the base of the penis albuginea citrate lead staining was observed in the transmission electron microscope TEM-1 200EX. 3, Application Realtime PCR, Western blot detection rats corpus cavernosum connexin 43 (CX43) expression. Results: 1, erections and erectile rate, diabetic group treated rats was significantly decreased compared with the control group, the difference was statistically significant (erections) or very significant (Erectile rate). 2 diabetic model group and model treated rats penile tissue ultrastructure compared with the control group changed significantly. The significant difference between the two groups of diabetic ED group, the treatment group compared with the model of endothelial cell damage, and smooth muscle changes in model group significantly. 3, the lowest diabetes model rat corpus cavernosum CX43 gene expression, the treatment group than in the model group (P <0.001), both of which are significantly lower than the control group (P <0.001). Western blot results showed that the same as the Real-time PCR results, CX43 protein in the treatment group than the diabetic group. Conclusions: 1 of CX43 gene and protein expression reduced important mechanism of erectile dysfunction caused by diabetes. Insulin therapy to improve the expression of the rat corpus cavernosum CX43 has an important role in the third part of Age with diabetes on erectile function in synergistic impact Objective: To study increasing age and diabetes on erectile function synergistic impact. Method: 1,3-month-old and 18-month-old experimental animals were randomly divided into two groups, each group is divided into an experimental group of 40 (including diabetic model group (n = 20), 20 model treatment group) and 20 control group. 12 weeks after injection of apomorphine (APO, 80μg/kg) was observed after the rats with or without an erection of the penis, penis erections. Glans hyperemia and the end of the penis body for erectile. 3, the two groups of rats were 12 weekend off were sacrificed, dissecting penile tissue. Citrate lead staining was observed in the transmission electron microscope TEM-1 200EX. 4, respectively. Application Realtime PCR and Western blot detection of each group rat corpus cavernosum gap junction protein expression of 43 (CX43). Results: 1 diabetes month-old rats (1) erections and erectile rate in the case of the same months of age, compared to the differences between the model group and the control group, the treatment group and the control group with significant statistical significance. (2) compared with the control group change is obvious, significant differences between the two groups of diabetic model group and the model treatment group in the 6-month-old rats group, diabetic model group and model treated rats penile tissue ultrastructure. In the 21-month-old rat group, diabetic model group and model treated rats penile tissue ultrastructure compared with the control group change significantly, no significant differences between the two groups of diabetic model group and model treatment group. (3) real-time PCR results in 6-month-old group, the Ct value of the control group, 22.5 ± 2.3, the treatment group was 25.9 ± 1.3, DM model group was 28.1 ± 2.7, statistically different among the three groups, P = 0.001 pairwise comparison among the groups, the difference between the DM model group and the control group, the treatment group and the control group was statistically significant P values ??were 0.001,0.001, but the difference between the DM model group and treatment group statistically significance, and a P value of 0.018. The group Ct value of the control group, 23.6 ± 3.5, the treatment group was 24.6 ± 2.6, DM model group was 25.1 ± 3.2, statistically different among the three groups, P = 0.001, pairwise comparisons among the groups, DM model group and the control group, the difference between the treatment group and the control group was statistically significant P values ??were 0.004,0.012, but no statistically significant difference between the DM model group and treatment group, a P value of 0.316. Western blot results show, the same as the Real-time PCR results of CX43 in protein expression in the 6-month-old group, the control group than the treatment group and the DM model group, the DM treatment group is higher than the DM model group than obvious. 21 group, the control group than the treatment group and DM model group, the expression was not significantly different between the the DM treatment group and the DM model group. 2 diabetic rats (1) age erectile erectile erectile rate, 21-month-old rats in model group decreased significantly compared with the June group difference was statistically significant (erections ) or very significant significance (Erectile rate). (2) real-time PCR results, 21-month-old group of diabetic rat corpus cavernosum CX43 gene expression is lower than the June group. 21-month-old diabetic treatment group rat corpus cavernosum of CX43 gene expression is also lower than the group in June. (3) Western blot results show, Real-time PCR results, the model of diabetes and the treatment group rat corpus cavernosum CX43 protein expression increased with age reduce. Conclusion: increasing age and diabetes are important factors that cause erectile dysfunction, both have a synergistic effect; 2. Increases with age, the role of insulin therapy for diabetic erectile dysfunction improve gradually reduced.