Dissertation > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Coronary arteries ( atherosclerosis ),heart disease (CHD)

Shandong coastal populations apolipoprotein gene cluster haplotypes A5/C3 characteristics and coronary heart disease study

Author HanTongLiang
Tutor KangWeiQiang;ZhaoZhongTang
School Shandong University
Course Epidemiology and Biostatistics,
Keywords Coronary heart disease Abnormal lipid metabolism Apolipoprotein A5 Apolipoprotein C3 Gene polymorphism
CLC R541.4
Type Master's thesis
Year 2011
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Plasma lipoprotein apolipoprotein is the aim of the protein component. Plasma lipid transport due to its key role in, and thus the artery atherosclerotic disease have a major impact. This study was to analyze the relationship between non-kin coastal areas of Shandong Han ApoA5/C3 gene cluster SNPs and haplotype characteristics, to investigate coronary artery disease (CAD) in patients with apolipoprotein (apolipoprotein, Apo) A5 and C3 gene variants with Crown heart disease and abnormal lipid metabolism correlation. Analysis of multi-locus SNP gene cluster ApoA5/C3 statistical variations and linkage disequilibrium and haplotype comparison; different chromosomes based on previous multi-SNP gene apolipoprotein collaboration with the formation of coronary artery disease susceptibility effects and mechanisms of gene ApoA5 SNPs with hypertriglyceridemia early clinical detection index, exploring ApoA5 SNP3 (-1131T gt; C) may be independent of coronary heart disease susceptibility genes. Methods Polymerase chain reaction - restriction endonuclease fragment length polymorphism analysis, combined with automatic sequencing, randomly selected non-relatives in Shandong Han patients with coronary heart disease and 275 cases of abnormal lipid metabolism (mean age 59.2 ± 9.8 years) , as well as age-and sex-matched healthy control group were 289 cases (61.6 ± 10.7 years), testing sites include: ApoA5 SNP3 (-1131T gt; C), S19W (C56C gt; G), SNP4 (-12238T gt; C) and ApoC3 (-482C / T); ApoC3 form and ApoA5 and haplotype frequencies as well as significant changes in the CAD multilocus SNP in joint analysis, simultaneous measurement of serum lipid levels in patients with coronary heart disease results ApoA5 SNP3-113lTT bit Point prevalent than normal TC genotype (P lt; 0.05); coronary heart disease ApoA5 SNP3-ApoC3-482C / T gene linkage disequilibrium between a significant difference (P lt; 0.05); coronary heart disease ApoA5 * 3-ApoC3-482T haplotype blocks than the control group (P lt; 0.05); TG levels in the CHD group ADoA5 SNP3 TC-type group was significantly higher than the other two genotypes (P lt; 0.05), while ApoC3-482CT TG levels compared with genotype CC and TT group also increased significantly (P lt; 0.05). Conclusion This study shows ApoA5 SNP3 - 1131TT-ApoC3-482TT and SNP4 TC-ApoC3-482CT linkage disequilibrium, ApoA5 SNP4 and ApoC3-482 shows the relationship with coronary heart disease have significantly susceptible; coronary heart disease ApoA5 * 3-ApoC3-482T frequency of haplotype blocks were significantly higher SNP loci influence of genetic linkage disequilibrium and haplotype blocks constituted of coronary heart disease susceptibility gene genetic effects; has ApoA5 SNP3 TC and ApoC3-482CT genotype in patients with coronary heart disease are associated with high TG levels, two genotypes of the impact of variability on TG relatively independent; haploid genetic susceptibility to coronary heart advantage is an important factor.

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