Fine Mapping and Genetic Dissection of QTL for Yield Traits on the Short Arm of Rice Chromosome 6
|School||Chinese Academy of Agricultural Sciences|
|Course||Crop Genetics and Breeding|
|Keywords||Rice Quantitative trait loci Yield Traits Fine mapping Residual heterozygous|
In the early Zhenshan 97B / the Miryang 46 remaining heterozygotes (residual heterozygous line, RHL) derived F2: 3 population the yield QTL mapping and decomposition, found its range of RM587-RM19784 on the short arm of rice chromosome 6 The top area of ??grains per panicle per panicle and grain yield per plant, with a significant role in the central region of grains per panicle per panicle, seed setting rate, grain weight and yield per plant with role and there may be more than one QTL. On this basis, the research from RHL-F3 groups screened four heterozygous interval submitted Diego's RHLs of heterozygous range for RM587 RM19410, RM587-RM225, RM19410-RM6119 and RM6119-RM402. Apply its F2: 3 groups, as well as secondary RHL derivative groups, including two sets of overlapping the heterogeneity intervals smaller F2 population, 5 sets of near-isogenic lines and chromosome segment substitution lines the regional control the short arm of chromosome 6 per panicle and per panicle QTL fine mapping QTL controlling yield traits of the central region of the genetic decomposition. The main results are as follows: 1. Application 3 sets of RHL-F2: 3 groups, as well as two sets of secondary RHL derivative groups to control the range of RM587-RM6119 per panicle per panicle total grains and yield per plant QTL mapping the 26.3 kb RM19410-Si2940 interval. Applications in the range of RM587-RM6119 heterozygous interval submitted the three RHLs Diego selfing produced three sets of F2: 3 populations were detected within the target range to the control of grains per panicle per panicle and individual yield QTL are additive, Zhenshan 97B allele from the synergies, and its mode of action and effect direction with a previous study detected QTL and their genetic mode of action. Therefore, it can be considered a target range of memory in a common QTLs regulated through the control of grains per panicle and panicle yield per plant. Compare three RHLs in the heterozygous range as well as on both sides of the target range coverage possible reorganization interval was heterozygous, three sets of population control per panicle QTL mapping of approximately 125.1 Kb RM19407-RM19417 chromosomal region. Subsequently, new developments in RM19407 and RM19417 a SSR and four InDel-mark, and the application of the two sets of secondary RHL F2 groups to further the QTL mapping 26.3 kb RM19410-Si2940 interval. The region has a total of three candidate genes. RM6119-RM6779 interval applications covering a RHL-F2: 3 groups, and its derivative 5 sets of near-isogenic lines, and 6 set of chromosome segment substitution lines decomposition 4 QTL controlling yield traits, which are located 96.1- 860.5 kb region. Application heterogeneity interval RM6119-RM402 FM10 F2: 3 groups QTL analysis of yield traits, indicating that the range does not work on the heading stage, but yield traits, especially grain yield per plant and per panicle, has an important role . Subsequently screened in the F2 population obtained five heterozygous interval smaller and submitted the secondary RHLs Diego. 5 sets of near-isogenic lines and chromosome segment substitution lines were constructed from the the RHL F2 population derived. RM1163-RM19652 interval decomposition of a QTL controlling yield per plant, while controlling per panicle per panicle grains and single allele effects analysis and overlap the recombinant chromosome segment substitution lines Plant production QTL control per panicle QTL and a QTL controlling per panicle and grains per panicle. These QTL are located 96.1-860.5 kb region. All QTL are performance-based additive, which is located in RM19521-RM111 interval while controlling per panicle per panicle and yield per plant QTL alleles from Miryang 46, the rest QTL alleles were from Zhenshan 97B.