The Expression Level of Growth Factor Beta-1 in Induced Sputum of Coal Miners
|Course||Epidemiology and Biostatistics,|
|Keywords||Coal mine workers Induced sputum Transforming growth factor - beta1|
Background pneumoconiosis caused by exposure to dust production is the most serious occupational diseases in the incidence of occupational diseases in China. Due to the lack of specific means for the treatment of pneumoconiosis, pneumoconiosis mortality is extremely high, and therefore to select and monitor to harm sensitive indicator of early workers exposed to dust from the dust-exposed high-risk groups early, delaying the progression of pneumoconiosis patients and extend the life of great significance . In recent years, the respiratory tract induced sputum technique is widely used in the diagnosis and treatment of diseases such as asthma, attaches great importance to growing international pneumoconiosis researchers. Induced sputum analysis is expected to be used as a method for regular monitoring of the health status of workers exposed to dust means to supplement and complete the traditional monitoring methods, contribute to the diagnosis of occupational lung disease. Transforming growth factor p plays an important role in the regulation of lung fibroblast proliferation, division and collagen synthesis and degradation process are highly concerned about the relationship with pulmonary fibrosis. Which, TGF-β1-induced fibrosis strongest body damage repair, remodeling has become the focus. The purpose of this study, respiratory induced sputum technique induced dust exposure levels of workers deep lung sputum, simultaneous determination of the expression level of TGF-beta1, sputum analysis and workers exposed to dust levels and pneumoconiosis lung tissue pathological changes related to supplement and complete occupational history inquiry and environmental monitoring methods and other traditional occupational disease detection methods, provide new ideas and means to further strengthen and improve the the early dust hazard prevention and control measures. Method 1. The respiratory induced sputum: non-pneumoconiosis workers (including divided into different dust exposure of workers and non-workers exposed to dust) 115 and pneumoconiosis patients and their corresponding control 32 respiratory induced sputum. 2 sputum cell counts and classification: first with dithiothreitol treatment sputum, and then hematoxylin - eosin staining to count the total number of cells and differential count, inflammatory cells to detect the different subjects, analysis whether there are differences among the groups sputum cytologic features. Sputum cells within the target gene of TGF-beta1 mRNA expression levels of detection: to extract the sputum cell total RNA, reverse transcriptase PCR reaction system using real-time quantitative PCR of TGF-beta1 mRNA expression levels for the first strand cDNA preparation. Results All subjects can accept sputum induction, a success rate of 98.6%. Pale or yellow, viscous sputum, workers exposed to dust and sputum of patients with pneumoconiosis occasional black coal dust, about 2 ml of sputum. Squamous epithelial cells (8.94 ± 3.59)% cell viability (78.32 ± 6.50)%. 2 workers exposed to dust for more than 10 years the proportion of macrophages (50.53 ± 11.12)%, higher than that of other working-age group (P lt; 0.05). Dust exposure of workers more than 10 years of TGF-beta1 mRNA expression level is 1.21 times the corresponding control (P lt; 0.05). 4.1 pneumoconiosis, Phase II and Phase III pneumoconiosis patients TGF-beta1 mRNA expression levels were 1.53 times, 1.52 times (P lt; 0.05) Conclusion 1. Respiratory proportion of macrophages may be involved in the pathogenesis of pulmonary fibrosis process; induced sputum method can be applied to the monitoring of the dust-exposed workers sputum cytology changes. Dust exposure of workers in more than 10 years, patients with pneumoconiosis I, II and III of pneumoconiosis patients compared with the respective control, TGF-beta1 mRNA expression level of content increased, indicating that TGF-beta1 may be involved in the occurrence of pneumoconiosis development, and can be used as a screening high-risk populations and biological monitoring indicators.