Effects of Several Reproductive Hormones on Apoptosis of Ovarian Granulose Cells of Yaks in Vitro
|School||Gansu Agricultural University|
|Course||Clinical Veterinary Medicine|
|Keywords||Yak Granulosa cell culture Apoptosis , proliferation Steroid hormone secretion Reproductive hormones|
Follicular atresia is the mammalian ovary very common natural phenomena. The essence of follicular atresia follicular cell apoptosis, granulosa cell apoptosis is the direct cause of follicular atresia. It has now been confirmed, granulosa cell apoptosis is not only associated with the apoptosis-related genes, but also by the regulation of many cytokines and Caspases family, is also affected by the impact of cell junctions and extracellular matrix, more importantly, is the first and foremost by the reproductive hormones networked control . Currently involved in research data on reproductive hormonal regulation of granulosa cell apoptosis also limit people to understand and master hormone control of ovarian function. Based on this, the study's selection of yak granulosa cells of preovulatory follicles as the research object, add Mel affect ovarian function in vitro culture system, GnRH, FSH, LH, E 2 and P 4 several important reproductive hormone, using flow cytometry, HE labeling method vitro granulosa cell apoptosis rate and mitotic index, and radioimmunoassay method for detection of progesterone and estrogen in the culture medium. changes in hormone concentrations and confirmed apoptotic morphology with cell ultrafine microstructure. The research results show that the yak granulosa cells cultured in vitro, and the ability to secrete progesterone and estrogen. FSH, Mel, P 4 and LH has a to promote granulosa cells enter mitosis proliferative state. Granulosa cells cultured in vitro spontaneous apoptosis the FSH, Mel, P 4 and E of 2 can be suppressed; FSH strong inhibition of GnRH and Mel impact, and will not be. Mel role of inhibition of apoptosis is highly vulnerable to GnRH, FSH and LH, but not significantly inhibit or superimposed effect. GnRH can induce apoptosis of granulosa cells cultured in vitro, its pro-apoptotic effect of FSH completely reversed; while Mel, E 2 and P 4 only partially alleviate GnRH proapoptotic role. FSH, Mel, LH and E of 2 can promote granulosa cells to secrete progesterone; Mel and E 2 simultaneously superimposed to promote the role of progesterone secretion. GnRH strongly inhibit granulosa cells cultured in vitro secretion of progesterone and its inhibitory effect is almost independent of FSH and LH influence; Mel showed only partially alleviate the inhibitory effects of GnRH, but there is no reversal. E 2 can not only completely reversed the inhibition of GnRH and thus with GnRH superimposed promote granulosa cell secretion of progesterone. FSH, LH, Mel, GnRH and P 4 can promote granulosa cells cultured in vitro secretion of estrogen; Mel can promote granulosa cells secrete estrogen inhibit P 4 GnRH and FSH or LH together inhibit granulosa cells secrete estrogen.