Dissertation
Dissertation > Biological Sciences > Zoology > Animal ecology and zoogeography

Molecular Ecology Studies on Genetic Diversity and Differentiation of Common Krill in China Seas

Author GuoDongZuo
Tutor LiShaoZuo
School Xiamen University
Course Marine biology
Keywords Pacific krill Wide turnover fake krill China fake krill Allozyme Genetic Diversity Genetic differentiation
CLC Q958
Type PhD thesis
Year 2003
Downloads 294
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Krill is a class of large zooplankton, quantity, wide distribution, is an important zooplankton taxa, one of the marine ecosystem plays an important role, especially in the Southern Ocean, is to maintain marine primary producers and higher One of the major aspects of nutrition-order; Meanwhile, krill is the main bait fish in many economies in recent years, emerging as krill fishery resources is causing people's attention. The world's oceans krill nearly 90 kinds, China has so far offshore krill record 2 families, 7 genera, 47 species of which the Pacific krill, krill long forehead, wide forehead false false krill and krill as the main advantages of China kind. Their ecological characteristics and distribution centers vary as currents and water masses of indicator organisms. In this paper, polyacrylamide gel electrophoresis, through allozyme analysis of China's offshore Pacific krill, krill and China wide forehead false false krill population genetic diversity and genetic differentiation, in order to further understand the origin and krill Phylogenetic provide experimental evidence, but also for the conservation of marine plankton provide basic information. (A) Pacific krill genetic diversity and differentiation of: 1. This paper analyzes the Yellow Sea two voyages, eight stations in the Pacific krill populations in seven enzyme systems were detected nine enzyme sites, namely: α amylase (a locus, 3, etc. alleles), R amylase (a locus, two alleles), esterase (three loci, Est5, Est6 and Est7, except Est7 single state outside, Est5 and Est6 have two alleles gene), malate dehydrogenase (1 locus, two alleles), malic enzyme (a locus, two alleles), lactate dehydrogenase (1 locus, 4, etc. allele), glucose phosphate aminotransferase (1 locus, 5 alleles). 2. In addition to E1 stations are not due to the small number of samples for genetic analysis, the remaining seven stations in the Pacific krill populations have higher genetic diversity: each station the average effective number of alleles per locus A e is 1.2845 ~ 1.4165, expected heterozygosity H e is 0.1457 ~ 0.2271, P percentage of polymorphic loci was 42.86 ~ 87.50%; All stations Pacific krill total polymorphic loci Percent P = 88.89%, expected heterozygosity H e = 0.3140 ± 0.2552, A e = 1.6628 ± 0.6585. 3.7 stations Pacific krill higher degree of genetic differentiation among populations, G st = 0.4837, illustrate the genetic diversity of the total population 48.37% from all stations between genetic variation, 51.63% belong to the genetic variability within stations, with the F statistic F st = 0.5123 basically the same. Gene flow between stations N m = 0.2380, indicates that gene flow between each station is limited, reflecting each station exists between isolation. China's coastal krill common genetic diversity and differentiation of molecular ecology genetic distance between each station is D two 0.3383 (0.0032 a 0.7641), genetic identity I = 0.7482 (0.4658 a 0.9968), cluster analysis showed that: yellow, Donghai Pacific krill can be divided into two populations, one in the Yellow Sea cold water mass from the center of the group structure, the other by the edge of the Yellow Sea and the East China Sea cold water mass groups of components. Currents, water masses as well as differences in habitat selection may lead to Yellow Sea and East Pacific krill biochemical genetic differences between populations of the main reasons. (Two) Amount false krill wide genetic diversity and differentiation of a paper off the coast of the East China Sea and South China Sea 2 stops bits wide turnover false krill populations were analyzed in the detection of nine enzyme systems were detected 11 enzyme loci: aspartate aminotransferase (l loci, two alleles), alkaline phosphatase (two loci, A and A plus 2 plus have two alleles), R amylase (l loci, two alleles), esterase (two loci, Es clever and Est7 have two alleles), malate dehydrogenase (l loci, 3 allele), malic enzyme (l loci, two alleles), lactate dehydrogenase (l loci, four alleles), glucose phosphate aminotransferase (l loci, 3, etc. allele); a single state amylase. Tokai E7 stations which are not analyzed for ALP and AAT, Est7 stations have only E7 expression. 2.2 Station-bit wide turnover false krill highest level of genetic diversity: each station the average effective number of alleles per locus A. A 1.6534 to 1.3791, expected heterozygosity He is 0.224) 0.3269, the percentage of polymorphic loci P is 70.00 a 75.00 percent; all stations bit wide total amount of fake krill percentage of polymorphic loci P two 90.91 percent, expected heterozygosity He = 0.3336 ± 0.1 961, A. = 1.6310 ± 0.1509. 3.2 Station-bit wide turnover false krill higher degree of genetic differentiation among populations, Gs, = 0.2804, described the genetic diversity of the total population 28.04% from all stations between genetic variation, 71%% are within stations genetic variation, close to the F statistic Fs, two 0.3 cited O. Gene flow between stations gung two O, 4832, indicates that exist between stations isolation, gene flow is limited. The genetic distance between stations is D two 0.2749, genetic identity I = 0.75%, results showed that: E7 stations and wide forehead 06A stations were fake krill represent different geographic populations, spatial segregation is the East, the South China Sea wide forehead False biochemical genetic differences between populations of krill main reason. (Three) China genetic diversity of a false krill Xiamen Hong Kong and China in the detection of false krill 9 enzyme systems were detected 10 enzyme loci: China's coastal common genetic diversity and differentiation of krill Molecular Ecology Research aspartate aminotransferase, a amylase, intoxicated enzymes and malic enzyme are l loci with two alleles; lactate dehydrogenase and malate dehydrogenase (l loci, 3, etc. allele), glucose phosphate aminotransferase (l loci, four alleles); alkaline phosphatase (two loci, there months plus two alleles); AIPZ and R amylase as a single state. China fake krill genetic diversity minimum, average effective number of alleles per locus A. Two 1.2624 ± 0.3363, expected heterozygosity He is 0.1 \Division boundaries obvious.

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