On Separation and Combination of Detoxicating Microbes, Detoxicated Theory and Applied Effect in Biology Detoxicating Method of RSM
|School||Gansu Agricultural University|
|Course||Crop Cultivation and Farming System|
|Keywords||Rapeseed Detoxification Microorganism Enrichment Glucosinolates Distant complex Tenebrio|
Fermentation rapeseed detoxification method refers to the microbial detoxification method. Number of microorganisms, many kinds of fast growth, easy to cultivate, and the enzyme system is complex, can be broken down almost all natural substances. Microbial agents rapeseed virus-free, has the following characteristics. First, the enzyme system is complex, diverse decomposition glucosinolate myrosinase, capable of hydrolyzing the midst of glucosinolates. Second, the decomposition of the intermediate product of complex enzymes can complete decomposition and synthesis of microbial own material. Rapeseed other anti-nutritional factors can also be broken down by microorganisms, such as phytic acid, tannin, cellulose, sinapine greatly change the taste of rapeseed, improve rapeseed nutrients utilization . Fourth microorganisms using its own metabolism, can produce of fragrance substances or flavoring substances precursors, to improve rapeseed after detoxification fragrance, so the authors suggest that microbial detoxification method is a promising detoxification method, it should be the majority of development. By year indoor microbial separation, purification and detoxification test and one year of breeding experiment to study the the rapeseed detoxification microorganisms in the soil enrichment and screening, primary forty kinds of microorganisms in pure rapeseed cake detoxification, the genus genus mold compound rapeseed detoxification, screening compound FDMRM-detoxification effect test and breeding experiment with FDMRM-1 production Detoxicated. The obtained results are as follows: 1. The use of rapeseed fermentation substrate, soil on Class A, B class soil C soil rapeseed detoxification microbial enrichment. Class A soil enrichment process rapeseed detoxification results, the GS virus-free rate was 98.4%. The overall detoxification effect is a Class A soil class B soil C class soil. The total number of bacteria in the Class A soil after enrichment, proliferation 4.5 times 4.0 times; proliferation in the Class B soil; proliferation 3.4-fold in the Class C soil. Mold the total number of Class A soil after enrichment and proliferation of 10.01 times; proliferation in the Class B soil 11.71 times 11.66 times; proliferation in the Class C soil. The total number of actinomycetes in the soil of Class A proliferation 1.5-fold, 2.8-fold proliferation in the Class B soil; proliferation by 3.3-fold in the Class C soil. The the yeast total number of Class A soil proliferation 4.0 times 7.5 times; proliferation in the Class B soil, proliferation nine times in the Class C soil. Based on changes in the number of micro-organisms, the preliminary view that the GS detoxification and virus-free number of microorganisms was positively correlated with detoxification role of the fungal detoxification role of bacteria> actinomycetes role in detoxification. Isolated and purified from the enriched soil forty kinds of the microorganisms for FDMRM developed. 2. The main toxic component of glucosinolates in the rapeseed variety of structures, a variety of structures coexist in the same sample. Different varieties of rapeseed cake glucosinolate content and composition of the type, there are differences in the glucosinolate content and composition of the different samples of the same varieties of rapeseed cake, this is because the the rapeseed cake glucosinolate content and composition vary depending on not only the genetic characteristics of rapeseed , but also by the oilseed rape cultivation conditions, the rapeseed storage conditions, oil processing conditions and rapeseed storage conditions. Is it glucosinolate for a rapeseed varieties, is complex. 3. Microbial degradation of glucosinolates may contain a certain class or certain types of myrosinase, it is only one or a few glucosinolate degradation the rapeseed in structure and their adaptation the structure incompatible glucosinolates can not be degraded . A micro-organism glucosinolate degradation rate of the highest value (or theoretical), glucosinolates should be corresponding to the percentage of the total glucosinolate. Mustard enzymes secreted by different microorganisms, GS degradation rate in the same sample of rapeseed. GS degradation rate of the same micro-organisms in different rapeseed samples (difference due to different samples and sulfur II). The essence of the rapeseed detoxification microbial compound is a myrosinase complex, due to the increase in the compound after mustard enzymes type structure sulfur II, the corresponding proportion in the total sulfur II increase the degradation rate of sulfur two will increase accordingly. Within the genus mold compound, GS degradation rate increased, the case of fungal compound, GS degradation rates have greatly improved. Distant microbial compound to achieve a more perfect detoxification effect, GS degradation rate of over 99%, phytic acid, monolithic, a great degree of cellulose degradation. Myrosinase structure and properties of different microorganisms have different degrees of overlap. Where the type of structure in a microorganism, A myrosinase is abc three myrosinase structure type is the ABD in acetic microorganism overlap ab, the sulfur two degradation rate difference in the on the ab percentage basis. And d the difference. Microbial genetic relationship farther, myrosinase larger structural differences, myrosinase structure of the less overlap, or more light, distant microbial compound theoretical basis. The distant multi-microbial compound Geotrichum, four micro-organisms such as yeast, the GS virus-free rate is not high, but can be bacterial protein synthesis of inorganic nitrogen, making rapeseed detoxification protein content increased to 39.2%, thus improving the nutritional value of the virus-free cake. . 1 FDMRM the detoxification agents (distant microbial compound) microcosms of rapeseed biological detoxification process. During the fermentation, the fermentation temperature of the environment type, the type of state change of the pH value of the fermentation process, the temperature variation of the type of fermentation product. Ambient temperature at 25 ℃ low-grade temperature changes than ideal, GS degradation rate of 0.8%. Proposed rapeseed detoxification alternate microbial culture isolation method, which is separated mold Czapek medium RSM alternating medium culture: separation of bacteria with beef extract peptone medium RSM medium alternately culture; separation of yeast bacteria wort medium RSM medium alternately culture, with half the times. With the fermentation FDMRM production of virus-free bread instead of 100% soybean meal added to pig feed for pig fattening, daily gain of 5079, feed conversion ratio 3.30:1, and control (by adding soybean meal) feed to feed the effect is very close (P gt; 0.01). Month with normal body quality and visceral weight in fattening pigs feed virus-free cake can completely replace soybean meal, can greatly reduce feed costs. 7. Used FDMRM a fermentation production of virus-free cake breeding Tenebrio three successive generations, no teratogenic effects. Virus-free cake to add an unlimited amount of Tenebrio feed, according to the ratio of feed OK. Can reduce feed costs, but also improve the nutritional and accelerate the growth rate of larvae increased fecundity, can significantly improve the effectiveness of insect rearing.