Dissertation
Dissertation > Medicine, health > Chinese Medicine > TCM Internal Medicine > Modern medicine, internal diseases

Association of Angiotensin-Converting Enzyme Gene and Coagulation Factors Ⅶ Gene Polymorphisms with Blood Stasis Syndrome in Coronary Heart Disease

Author MaoYiLin
Tutor YuanZhaoKai
School Hunan College of Traditional Chinese Medicine
Course Diagnostics of Chinese Medicine
Keywords Coronary heart disease Stasis syndrome Pedigree analysis ACE gene polymorphism F Ⅶ gene polymorphism
CLC R259.4
Type PhD thesis
Year 2003
Downloads 163
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Objective To investigate the Coronary Heart coagulation factor VII (F VII) gene polymorphism, angiotensin-converting enzyme (ACE) gene polymorphism. Method 1. Literature: System ancient and modern literature, to understand the characteristics of distribution of Coronary Heart Disease and its research progress. Epidemiological studies of the genetic predisposition of Coronary Heart: the use of pedigree analysis of 54 cases of coronary heart disease patients with blood stasis syndrome and 54 healthy family pedigree genetic analysis, detection of first-degree relatives of the incidence of genetic rate and three kinds of parents of children of mating types incidence of relative risk (RR) and its 95% confidence interval (95% CI). 3. Use of the polymerase chain reaction (PCR) technology ACE genotype blood stasis of coronary heart disease, non-blood stasis syndrome patients and healthy people, allele frequency and serum angiotensin Ⅱ (Ag Ⅱ), nitric oxide (NO) indicators for detection of vascular endothelial function and plasma endothelin (ET), a comprehensive analysis to explore the ACE gene insertion / deletion (I / D) polymorphism and coronary heart blood stasis relationship. 4. Use of the polymerase chain reaction - restriction fragment length polymorphism analysis (PCR-RFLP), blood stasis of coronary heart disease, non-blood stasis syndrome patients and healthy people F Ⅶ genotype and allele frequency detection while detecting activity of F Ⅶ (F Ⅶ C), antithrombin III (AT III), platelet alpha granule membrane protein (GMP-140), tissue-type plasminogen activator (t-PA), plasminogen activator The indicators reflect coagulation and fibrinolysis inhibitor -1 (PAI-1), and so on, comprehensive analysis to explore the relationship between F Ⅶ gene MspI polymorphisms with coronary heart blood stasis. Results. Literature: stasis syndrome is the most common clinical coronary heart disease TCM syndromes. The Coronary Heart existence coagulation system, anti-coagulation system, endothelial function and lipid metabolism disorders, and abnormal gene expression. The incidence of coronary heart disease with a variety of susceptible gene polymorphism. 54 cases Coronary Heart probands and 54 healthy people pedigree genetic analysis, the results lt; WP = gt; show that first-degree relatives of patients with Coronary Heart incidence rate of 7.62%, health The control group was 0.95%, the two groups have a significant difference (p lt; 0.01); the Coronary Heart genetic was 67.4 ± 6.89%; \the group RR value of 3.46, \Coronary Heart Disease may be a genetic predisposition polygenic witness. \Coronary Heart Disease and ACE gene polymorphism and vascular endothelial function Coronary Heart Disease DD genotype and D allele frequency was significantly higher than the non-blood stasis and healthy people (p lt; 0.01 or p lt; 0.05), and blood stasis and non-blood stasis, healthy people, throughout the observation object odds ratio (OR) were statistically significance. Coronary heart disease patients with BSS Ag Ⅱ, ET / NO detection value was significantly higher than the non-blood stasis and healthy individuals (p lt; 0.05, or p lt; 0.01), indicating that the DD genotype DD patients with BSS highest the stasis syndrome vascular endothelial function in patients with severely damaged. Coronary Heart Disease and F Ⅶ genotypes, allele frequency and coagulation, fibrinolysis indicators show that despite coronary heart disease in patients with blood stasis syndrome the M1M1 genotype and allele frequency higher than the healthy group and crown trend of heart disease non-blood stasis group, but the difference was not statistically significant sex (p gt; 0.05), likely due to the relationship of the number of samples in this study have not yet found Coronary Heart F Ⅶ gene the MspI polymorphism exists related sex. Plasma F Ⅶ C, GMP-140, AT Ⅲ coagulation the - anticoagulant indicators and reflect the fibrinolytic system activity of t-PA and PAI-1 activity detection found blood stasis and non-blood stasis, and the healthy control group, there was significant differences (all P lt; 0.01 or p lt; 0.05), the presence of coagulation, anti-coagulation and fibrinolytic system dysfunction, manifested Coronary Heart prothrombotic hypercoagulable state. Conclusions Blood Stagnation Syndrome of coronary heart disease is the most common type of clinical evidence. Blood Stagnation Syndrome may be a genetic predisposition \3.ACE gene polymorphism Department of Coronary Heart disease susceptibility genes. 4.F Ⅶ gene polymorphism may be associated with no significant correlation between the incidence of coronary heart disease blood stasis.

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