Study on Genetic Diversity of Alfalfa(Medicago Sativa L.) Germplasm in China
|School||Beijing Forestry University|
|Keywords||Chinese alfalfa Variety Resources Genetic Diversity Allozyme AFLP|
In this study, morphological, and other bit enzymes and AFLP and 40 copies of the material to the Chinese alfalfa samples, control 34 of the United States, France and Australia were the first pair from the three levels of the phenotype, proteins and DNA The relationship between genetic variation and its evolution and distribution of the resources of alfalfa varieties were analyzed, made the preliminary results of of alfalfa varieties Genetic Diversity distribution law. The study found that eight morphological traits variation, the alfalfa population genetic variation is very rich variation within populations tested material is greater than the variation between populations. In morphological traits, the first true leaf height having the highest level of variation, the coefficient of variation within populations of 26.40%, and the variation of more than 10% in the rest of the traits of the leaf aspect ratio indicator, up to 14.26%. The correlation analysis found that, in addition to the two indicators of leaf width and number of branches per plant, showed a significant correlation between the rest of the characters are. The principal component analysis showed that plant height factors and leaf length factors constitute the first factor, plays a leading role in all morphological variation sources, these two factors. Four kinds allozyme system LAP, EST, PGM and MDH starch gel electrophoresis analysis showed that nine loci and 28 codominant alleles detected in 22 Chinese and foreign alfalfa varieties (lines) material Communist China . Study each zymogram the monomer enzyme and the dimeric enzyme autotetraploid alfalfa enzyme law on the basis of Mendelian inheritance. The results show that, the alfalfa populations Allozyme analysis of the average proportion of polymorphic loci was 74.07%. The polymorphic loci allele frequency range of 0.788 to 0.003, with an average number of alleles on each locus was 2.73, significantly higher than the the foreign control population (0.785 to 0.002 and 2.43). The first application of AFLP and 12 provinces in northern China alfalfa distribution representative of alfalfa (Medicago sativa L.) population of 40 copies of material genetic diversity analysis. The results showed that 40 alfalfa populations AFLP marker map shows high polymorphism. From the screened AFLP primer combinations (are E35/M50, E35/M49, E38/M62 E40/M47), 177 clear banding, of which 97 were polymorphic, per pair of primers 44.3 with amplified polymorphism with the proportion of 54.8%. Chinese alfalfa populations using Nei's genetic similarity coefficient and genetic distance matrix of genetic distances range from 0.548 to 0.802, the clustering results show that the origin and distribution of more recent varieties (lines) with priority clustering trend.