Dissertation > Biological Sciences > Entomology > Insect Physiology

Studies on Nerves and Hormones Regulation in Gonad Development of Reticulitermes Aculabialis

Author SuXiaoHong
Tutor ZuoGengSi
School Shaanxi Normal University
Course Zoology
Keywords Termite Gonadotropin c-fos Estrogen receptor Immunocytochemistry
CLC Q965
Type PhD thesis
Year 2004
Downloads 146
Quotes 4
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Termites originated in the Cretaceous, so far there are 200 million years of history, is the world's oldest social insects. Has a complex quality differentiation and clusters of nesting characteristics of life, showing Isoptera extremely forward in the evolutionary road characteristics, so that can be a huge development, and biological characteristics of these evolutionary Insecta rare. Extremely wonderful and special biological characteristics, is considered to be the \Termites not only because of its wonderful and special biological characteristics, has been attracting a great deal of attention and interest, and endanger agriculture, forestry, water conservancy, transportation, financial services, chemicals, building construction due to its feeding behavior and even military installations. Therefore, the world of termites very seriously. The sharp lip termite (Reticulitermes aculabialis) affiliated Isoptera (Isoptera), nose the termites Division Rhinotermitidae) especially of building the kind of serious harm to the Northwest. To tip lip termite cytology, morphology and anatomy, methods such as immunocytochemistry, its grade differentiation the oogenesis microscopic structure, neural and hormonal regulation of gonadal development, neurohormonal minutes flying oncogene gene regulation, and conduct studies on the molecular mechanisms of the termite reproductive physiology and grade of differentiation, and provide a theoretical basis for its biological control. The results are as follows: 1. The sharp lip termite grade into reproductive ants and non-reproductive ants two categories. Reproductive ants including the primordial germ ants and complement the reproductive ants (long-winged black supplement type, short wing buds black supplement type, short the wing buds yellowish supplement type, the micro fin buds subtilis yellow supplemental type, no wing buds supplemental type); non- reproductive ants, including the soldier and worker ants. Flying the primordial germ ants by marriage breeding ants off wings formed after pairing; supplement the reproductive ants by not instars ants or ants differentiation; soldier from 4 years old worker ants differentiation; worker ants 2nd instar young ants. 2. Bulk the termites oogenesis microstructure research pointed lip, oogenesis is divided into the of oocyte differentiation period, growing season, and the previtellogenic period, and is subdivided into seven stages. Oogenesis, termites and other insects are basically the same. Vitellogenesis stage of oogenesis key: follicular cells are very active during vitellogenesis involved in the formation of the yolk, and may directly regulate the maturation of the egg. 3. The gonadotropin including follicle stimulating hormone (FSH) and luteinizing hormone (LH), and they are widely present in the vertebrate brain, promote gonadal development sperm mature and steroid hormone production. Invertebrates, insects, the presence or absence of FSH and LH, how the distribution has not been reported. Immunocytochemistry technology tip lip scattered the termite Reticulitermes aculabialis brain distribution of FSH and LH in positioning research. Immune response: of FSH immunoreactive nerve cells and nerve fibers distribution in the right lobe of the female reproductives nuptial flight before the brain's left lobe, right lobe and central; female last instar nymphs forebrain; the male reproductives nuptial flight before brain of the right lobe. LH immunoreactive material distribution in the female, the male reproductives nuptial flight before the brain left lobe. Worker ants and soldier ants did not show the presence of FSH and LH immunoreactive substances. The results showed that the FSH and LH in termite presence of brain developmental stage-specific, and may like vertebrates as involved in regulating gonadal development and sperm-egg mature. May adjust reproductives, soldier, worker ants produce. This is the first time that the presence and distribution of FSH and LH in termite brains, providing direct evidence to reveal the molecular mechanisms in insect sperm and oogenesis. 4. Proto-oncogenes C-fos is an immediate early gene, hormones and other stimuli to react for expression. Already know the mammalian sex occurred and maintaining the forebrain regions nerve cells c fos expression. The insect brain nerve cells c-fos expression whether related to their sexuality has not been reported. The nuptial flight termites sex. Immunocytochemical staining techniques of female and male tip lip scattered termite nerve cells in the brain c-fos expression study results show that: c fos expression the reproductives period have, but expressed most strongly in the nuptial flight, immune The positive substance locate the first cranial nerve nucleus and cytoplasm. The research results show that the c a f. The expression in the brain cells of the S in the when termites marriage fly with specificity, and may participate in the regulation of sex like in mammals as. This is the first time that the relevant brain regions of c-fos expression in their marriage flight before the termites to lay a foundation for revealing the molecular mechanisms of termites marriage flight. In this study, the first time that the termites marriage flight as their former brain neural activities are closely related; proto-oncogene c-fos in an important role in regulating gene expression in the forebrain nucleus related to sexual behavior. Our findings laid the groundwork for understanding of termites marriage flight adjustment mechanism. Estrogen receptor (ER) is a gonad development and refinement, the eggs mature sex hormone receptors in a direct relationship, whether there are estrogen receptors in insect gonads, whether as vertebrates as involved in their gonads the occurrence of mature sperm and ovum, has so far not been reported. The first time we used immunocytochemistry detected a sharp lip termite testes and ovaries in the presence of the estrogen receptor, as well as the precise positioning of the estrogen receptor in the testes and ovaries. The results show that the ER in the ovary and testis of the last instar nymphs are present, the immunoreactive substances positioning differentiation and growing oocytes and spermatogonia. Differentiation of oocytes ER localized in the nucleus; positioning in the growth stage oocytes, ER spermatogonia, ER localized in the nucleus in the cytoplasm and cell membrane; Therefore, you can wake up \This is the first time found that the presence of ER in the gonads of insects, for understanding insect sperm, eggs molecular regulation mechanism provides a theoretical basis. This study is the first to confirm the follicle-stimulating hormone [FSH) and luteinizing hormone (LH) in the brain of termite presence and distribution; demonstrated for the first time that the estrogen receptor (ER) in the role of termites sperm meets egg maturation; first to demonstrate termites before brain regions of the proto-oncogene c-fos expression in their marriage flight. And there may be a vertebrate regulate gonadal development as sperm mature and sexual behavior of molecular mechanisms. Our study was to evaluate the termite resistance

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