Dissertation > Medicine, health > Chinese Medicine > Chinese medicine practitioners of other disciplines > TCM neurology and psychiatry

Qingnao pills in the treatment of lacunar cerebral infarction and cerebral ischemia MARCKS phosphorylation regulation

Author HanZhenYun
Tutor WangYuLai
School Beijing University of Traditional Chinese Medicine
Course Traditional Chinese Internal encephalopathy
Keywords Lacunar infarction Stroke in Meridian Clear brain pills Multiple cerebral infarction MARCKS phosphorylation Cerebral protection
CLC R277.7
Type PhD thesis
Year 2004
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Background In recent years the popularity of stroke prevention and treatment, as well as prevention and control of risk factors for stroke, especially in the early aggressive treatment of hypertension and effective control of acute cerebrovascular disease increasingly lighter trend. With the imaging means of progress, especially in the wide application of MRI in clinical, lacunar infarction (Lacunar Infarction LI) greatly improve the diagnosis rate, and its proportion of cerebrovascular disease increased significantly. Under the leadership of academician in TRADITIONAL from \progress, but the incidence of early light-proof characteristics Syndrome, diagnosis and treatment and other studies to be perfect, so mild acute stage of stroke in Chinese medicine research evidence is even more important. LI mild clinical symptoms, the equivalent of the light stroke medicine card, card type diagnosed in the network through the card or card. Mild stroke in the clinical evidence occupies a very high morbidity. According to the \etc.), for the treatment of clinical observation of LI. Cerebral ischemia and hypoxia caused neuronal degeneration and cell death. Recent studies have found that excitotoxicity, calcium overload and its main mechanism of metabolic disorders, corresponding to produce a \Neuroprotective protein molecular level in the role has been recognized to some extent, different pathways through drug blocking cell death after ischemia, prolong cell viability, both for the prevention of high-risk patients, but also can promote neuronal function post- recovery, in order to achieve therapeutic purposes, while Chinese medicine has a multi-channel, multi-target protein in regulating brain after ischemia, interrupting the signal transduction pathway in toxicity, has become a research hotspot nerve cell protection. MARCKS (myristoylated alanine-rich C kinase substrate) is a protein kinase C (PKC)-specific substrate protein, PKC can regulate its phosphorylation, previous studies found that PKC-MARCKS signal transduction pathways, especially MARCKS phosphorylation dynamics and brain growth development are closely related, then the pathological conditions such as cerebral ischemia and hypoxia leads to cell death, loss of function when, MARCKS phosphorylation changes in the central nervous system has not yet been clearly reported; while the medicine in the state of cerebral ischemia on MARCKS phosphorylation whether the impact of change, no clear exposition. This study therefore proposed and validated these hypotheses, explore traditional Chinese medicine from the protein level ischemic protection mechanisms. Objective (a) through clinical observation, the incidence of acute phase LI characteristics, syndrome characteristics; 2 clear brain pills in the treatment of lacunar cerebral infarction and cerebral ischemia MARCKS phosphorylation regulation (2) for clearance Brain pills in the treatment of lacunar infarction preliminary clinical observation; (3) study multiple cerebral infarction rat hippocampus, cortex and p a MARCKs MARCKS expression changes, further observation Qingnao pills on MARCKS signal transduction pathway from the protein level study traditional Chinese medicine on acute cerebral ischemia protective mechanism. Methods Prospective clinical study part of the design plan, in accordance with recognized traditional Chinese medicine, Western medicine diagnostic criteria, developed inclusion, exclusion, exclusion criteria, randomized, parallel-group approach, the \citicoline treatment group and 33 cases of Aspirin plus citicoline in the control group, treatment for two weeks. efficacy evaluation using neurological deficit scores and clinical syndromes of TCM scoring method to develop and adopt a unified Case Report Form; based standards are as follows: ① lacunar infarction diagnostic standard reference 1995 Chinese Medical Association Fourth National Conference on Cerebrovascular Disease revised \diagnostic criteria 1996 State Administration of Traditional acute encephalopathy drafting a collaborative group of the \Syndrome research and clinical diagnosis of \Scale (NIHSS) on the basis of improved clinical research to fit LI experimental research component method using modified Kaneko established animal model of multiple cerebral infarction, the application of clear brain pills intervention for both controlled study with nimodipine; using optical microscopy and transmission electron microscopy after modeling pathological changes of hippocampus and cell ultrastructural changes, the application of immunohistochemistry and Western blot detection of immobilized MARCKS protein and its phosphorylation in rat hippocampal modeling and the expression of the cortex, and then observe its regulatory role Qingnao pills. above clinical and experimental research data were applied SPSS10.0 statistical software for analysis. Results (1) clinical findings during clinical trials included outpatient treatment Groups \% of the \The number of cases meet the standards and other indicators also conducted a baseline comparison; Under this premise, its efficacy indicators were evaluated. Two adverse events in this study is that the treatment group Chinese abstract one case, accounting for 1.52% in the control group, 0 cases. The treatment group occurred in one case of adverse reactions hyperviscosity, according to analysts may not be associated with taking the drug, and take measures to deal with continued medication. Comparative baseline in both groups under the premise of a well-balanced, come to the following findings: ① LI incidence shows that male and female incidence rate is about 3:2; aged 75 to 65 a highest incidence; onset between the ages of 35-45 nor rare, 45 an incidence between the ages of 65 total surveyed \

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