Expression of Cysteine Sulfinate Decarboxylase (CSD) in the Reproductive System of Adult Mice and Regulation of CSD by Estradiol and Progesterone in the Uterus
|School||China Agricultural University|
|Keywords||Cysteine ??sulfinic acid decarboxylase Taurine Estradiol Progesterone Reproductive system Mice|
Taurine is one of the mammalian reproductive system is the most abundant free amino acids, plays an important role in the reproductive system, reproductive system, whether synthetic taurine and detailed reports. Cysteine ??sulfinic acid decarboxylase (CSD) is a key enzyme in the taurine biosynthetic pathway, the study of adult mice as experimental animals, mainly used for RT-PCR, Western blot and immunohistochemistry chemical system of the CSD in CSD's expression and immunohistochemical localization in the male and female reproductive systems, CSD in mouse embryonic Peri variation, as well as estradiol and progesterone regulation in CSD expression in the mouse uterus. The main research content and results, including the following four aspects: First, the first demonstration of taurine in the reproductive system of female mice of synthase CSD expression at the mRNA and protein levels, indicating that the uterus, fallopian tubes and ovaries through CSD path synthesis and secretion of taurocholic acid. The immunohistochemistry study results show that, in the estrous cycle, CSD distribution in the epithelial cells of the uterus and fallopian tubes and a small number of stromal cells. Proestrus and estrus CSD in vascular endothelial cells and smooth muscle cells of the immune reaction than late and interphase. CSD is present in the ovarian granulosa cell layer and primary follicles to preovulatory follicles, oocytes, the zona pellucida and follicular expression CSD after ovulation luteal cells also expressed strong CSD. CSD's immune response gradually decreased with follicular atresia and luteal degradation in the follicular and luteal. Found was dynamic distribution of CSD in mouse embryos around the bed of uterus uterine expression patterns of the D1 and D2 and the normal estrous cycle distribution only in a small number of epithelial cells and stromal cells. From the D3, stromal cells also expressed strong CSD continued to D6; begins to weaken and gradually disappear from D7. CSD performance at the site of implantation sites D6 and D18 connected to the uterus and fallopian tubes, epithelial cells the CSD positive, a small number of stromal cells express CSD, and not pregnant mouse uterus CSD expression patterns. D4 morning injection of taurine transporter inhibitor β-alanine reduced D9 number of embryo implantation and endometrial thickness showed that taurine plays a certain role in embryo implantation. CSD evolution patterns in the mouse uterus that taurine regulatory role in embryo development and implantation of uterine receptivity. Of three ovariectomized mouse model to study the estradiol (E 2 ) and progesterone (P 4 ) regulation of the expression of the uterus in the CSD. Semi-quantitative RT-PCR, Western blot and immunohistochemistry chemical test results have showed that, ovary removal significantly with reduced uterine the CSD mRNA in and protein expression levels, each hormone treatment group are to restore the CSD's expression level, alone treatment group, E < most sub> 2 role; the separate injections P 4 and P 4 and E 2 co-injection of promoting stromal cells in CSD's expression. The control group ((P 4 hormone analog implantation processing experiments, continuous injection three days P 4 , the fourth day only injection P 4 sub>) 3 P4), the shape of the uterine cavity did not reach a state of receptivity for implantation of the embryo; continuous injection three days P 4 , the fourth day of simultaneous injection of E 2 and the P 4 in the experimental group ((P 4 ) 3 P 4 E 2 ), glandular epithelium and luminal epithelium, strong expression of the CSD, the shape of the uterine cavity, and the expression of CSD are close to the state of normal pregnant uterus. The control group and the experimental group to promote the CSD of expression in the stromal cells. CSD and ERα double labeling immunohistochemistry showed that the CSD and ERα main co-exist in the uterine glandular and luminal epithelial cells. These results indicate that estrogen may be through its receptors directly or indirectly involved in the regulation of the differentiation of uterine epithelial and stromal cells in the CSD level of expression as well as CSD, thereby regulating the taurine content changes. Fourth, study the expression and distribution of CSD in the male reproductive system in mice, the first time that the expression of the testis, epididymis, and vas deferens CSD mRNA and protein expression levels are elevated from the testis to the epididymis, vas deferens in the highest. CSD is mainly distributed in the testicular Leydig cells and epithelial cells of the output tube, epididymis, and vas deferens, and stromal cells. Expression from the epididymal head to smooth muscle cells and stromal cells of the epididymis and vas deferens around CSD. Sertoli cells within the seminiferous tubules and spermatogenic cells and sperm in the epididymis and vas deferens not express CSD. These results suggest that male reproductive organs synthetic taurine through CSD synthesis pathway.