Dissertation > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord

Experiment of Liposome Mediated Glial Growth Factor Gene Transfer in Vivo on Recovery of Spinal Cord Injury in Rats

Author WeiMeiYang
Tutor LiJiaShun;JiaLianShun;YuanWen;YeXiaoJian;DongYan
School Second Military Medical University
Course Surgery
Keywords Spinal cord injury Gene therapy Glial growth factor Liposomes
CLC R651.2
Type PhD thesis
Year 2005
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In this study, the glial growth factor (GGF) , the potential role of acute spinal cord injury (SCI) . Modified Allen method to create a model of the rat thoracic spinal cord injury , and RT PCR method to detect the changes in the timing of GGF mRNA expression after spinal cord injury in rats . Building with rats GGF2 cDNA recombinant plasmid pEGFP -N1 - GGF2 pIRES2- EGFP- GGF2 using cationic liposome -mediated recombinant plasmid pEGFP-N1-GGF2 and pIRES2-EGFP-GGF2 transfected rat spinal cord observed GGF2 expression in the rat spinal cord and its nerve function recovery after spinal cord injury in rats . The results show that the GGF mRNA expression early after spinal cord injury sustained reductions ; liposome -mediated recombinant plasmid in vivo successfully transfected rat spinal cord expression GGF2 play a catalytic role in nerve function recovery after spinal cord injury in rats . Conclusion: reduce potential GGF expression after spinal cord injury in rats with spinal cord injury oligodendrocyte cell death , or apoptosis , and SCI axon regeneration and remyelination difficult . The spinal cord of GGF2 gene transfection can promote recovery of neurological function after spinal cord injury in rats and its possible mechanisms to a GGF2 promote glial precursor cells and oligodendrocyte proliferation and survival of oligodendrocytes in the injured spinal cord and promote demyelination re- myelination of axons and axonal regeneration .

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