Dissertation
Dissertation > Medicine, health > Ophthalmology > Retina and optic nerve diseases > Retinal disease

Investigation of the Endoplasmic Reticulum Stress Associated Apoptosis in Retinal Ischemia-Reperfusion Injury in Rats

Author LiHuiLing
Tutor JiangDeYong
School Central South University
Course Ophthalmology
Keywords retinal ischemia-reperfusion injury apoptsis endoplasmic reticulum stress BiP antisense oligonucleotide XBP-1 CHOP GADD34
CLC R774.1
Type PhD thesis
Year 2006
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[Objective] To investigate the Endoplasmic Reticulum Stress (ERS) Associated Apoptsis in Retinal Ischemia-Reperfusion Injury (RIR) in Rats. [Methods](1) The model of RIR in rats were established by transiently elevated intraocular pressure (110 mmHg×60 min). Sprague-Dawley (SD) rats were divided randomly into control group and RIR group. The apoptosis of the retinal cells were evaluated dynamically by TUNEL.(2) To investigate the expression of BiP and XBP-1 indicative of induction of ERS in rat RIR. 120 SD rats were randomly divided into control group and RIR group. Each group was consisted of 5 subgroups (i.e. 3 h, 6 h, 12 h, 24 h and 72 h afer RIR, n=12). The transcription levels of BiP in the rat retina were dynamically detected by semiquantitative RT-PCR. Proteins of Bip and XBP-1 were detected by immunohistochemistry.(3) To investigate the role played by BiP in the rat retina after RIR

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