Dissertation
Dissertation > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Gastric diseases

The Approach to the Relationship between Pulmonary Infection and the Change of Gastric Acid and Bacterial Growth Following Aging

Author ZhuMing
Tutor WuBenZuo
School PLA Postgraduate Medical School
Course Geriatrics digestion
Keywords stomach hypochlorhydria bacterial overgrowth pulmonary infection aging
CLC R573
Type PhD thesis
Year 2008
Downloads 113
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AIM: To investigate the relationship of gastric pH value, gastric bacterial number, and pulmonary bacterial number of rats. To observe if the bacterial retrogradation exists, and if the bacterial growth can cause pulmonary infection in rats. To compare the reaction of the change of bacteria growth which caused by the increase of gastric pH value. To investigate the human gastric microflora, and the relationship of human gastric pH value, gastric bacteria number, atrophic gastritis, the infection of Hp (Helicobacter pylori), and other factors. To approach the relationship between gastric pH value, gastric bacteria and pulmonary infection.Methods: 1.40 Wister rats, among them 30 were 3month, 10 were 22month, were divided to 4 groups. Nonaging control and aging control group were gavaged with normal saline, nonaging omeprazole group were gavaged with omeprazole (15mg/kg/d), nonaging citric acid group drunk citric acid (0.1mmol/L) in addition to gavaged with omeprazole. The pH value were measured after 2w, and bacterial culture of gastric and lung tissue were done, the pathological sections of rat stomach and lung were also made.2. 32 Wister rats, among them 20 were 3month, 12 were 22month, they were divided to 2 groups respectively, one was omeprazol group, and the other was control group. Omeprazole group were gavaged with omeprazole (15mg/kg/d), control group were gavaged with normal saline. At the 4th day, all rats gavaged with Escherichia Coli (E.coli) labeled with enhanced green fluorescent protein (EGFP) to track bacteria translocation. At the 14th day, the pH value were measured, and bacterial culture of gastric and lung tissue were done, the pathological sections of rat stomach and lung were also made. Fluorescence microscope and plasmid enzyme cutting were used to identify the origin of the bacteria.3. 69 objects, 53 male (45 objects≥60yr, 7 objects < 60yr, 16 female (10 objects≥60yr, 7 objects < 60yr). We got gastric fluid and gastric tissue from gastroscope. The pH value were measured, and the culture of aerobic and anoxybiontic bacteria of gastric tissue were done. The identification of the bacteria was done by the means of 16SrRNA. We approached that if the patients were suffered from pulmonary infection.Results: 1.Compared with nonaging control rats, gastric pH value, number of gastric and pulmonary bacteria did not increase significantly; gastric bacteria number of nonaging citric acid group was different from nonaging control, but the pH value and pulmonary bacteria number did not increase significantly. Compared with nonaging control and nonaging citric acid group, gastric pH value, number of gastric and pulmonary bacteria of nonaging omeprazole group increased significantly. Positive correlation was existed in gastric pH value, number of gastric and pulmonary bacteria of rats. There was no obviously infiltration of infectious cells in all gastric tissue. The lung tissue of nonaging, aging control and nonaging citric acid group was similar, all slightly infiltrated with lymphocytes. 30% of nonaging omeprazole group had heavier infection in lung.2. In stomachs and lungs of rats, E.coli (Escherichia coli, E.coli) could be cultured. These bacteria could send out green fluorescent under fluorescence microscope. The bacteria isolated from stomach and lung had the same plasmid cleavage map. Compared with nonaging control, gastric pH value and E.coli number in stomach of nonaging omeprazole group increased significantly, and the number of lung E.coli were the same of control. Compared with aging control, the gastric pH value and E.coli number in stomach and lung of aging omeprazole group increased significantly; Compared with nonaging omeprazole group, the gastric pH value and E.coli number in stomach were the same, but the number of lung E.coli were higher. Positive correlation was existed in the gastric pH value, the number of gastric and pulmonary E.coli of rats. There was no obviously infiltration of infectious cells in all gastric tissue. The tissue of lung nonaging and aging control were similar, all slightly infiltrated with lymphocytes. 50% of aging omeprazole group had heavier infection in lung.3. Logistic regression indicated that the number of aerobic bacteria of gastric tissue related to the gastric pH value, and was independent of sex, age, diabetes, Hp infection, and atrophic gastritis. The number of anoxybiontic bacteria of gastric tissue related to the gastric pH value, diabetes, and Hp infection. The older, the more dangerous to have atrophic gastritis. Gastric pH value was independent of age, sex, diabetes, Hp infection, and gastritis type. Among 66 objects, 10 had pulmonary infection, they had higher gastric pH value or toke antacids. 69 bacteria strains were isolated from stomach. Among the aging, the common types were streptococcus, Escherichia coli, and Neisseria. Among the young people, the common types were streptococcus and Escherichia coli.Conclusion: 1. Positive correlation was existed in the gastric pH value, the number of gastric and pulmonary bacteria of rats. Follow the increase of gastric pH value, the infection of lung increased.2. After fluorescence microscope and plasmid enzyme cutting, we could confirm that the E.coli which contain pGEX-4T -1-EGFP could translocate from stomach to lung. No obviously infection could be seen in stomach of all rats. Compared with aging control, the number of E.coli in lung in aging omeprazole group was higher, and pulmonary infection was heavier.3. The number of aerobic bacteria of human gastric tissue related to the gastric pH value, the number of anoxybiontic bacteria of gastric tissue related to the gastric pH value, diabetes, and Hp infection. Among the aging, the common types in stomach were streptococcus, Escherichia coli, and Neisseria. Among the young people, the common types were streptococcus and Escherichia coli.

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