Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

The Effect of the Nephritis Xiaobaifang Therpy Proteinuria of Adriamcycin Nephritis Rats

Author WangLiFan
Tutor ZhangZuo
School Heilongjiang University of Traditional Chinese Medicine
Course Chinese medical science
Keywords Traditional Chinese medicine Doxorubicin-induced nephritis Proteinuria Nephrin CD2AP
CLC R285.5
Type PhD thesis
Year 2008
Downloads 228
Quotes 2
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Objective:To test if nephritis Xiaobaifang decreases proteinuria level in experimental animals and investigate its mechanism by biochemical and molecular biological analysis to provide the basis for its clinical application.Methods:We utilized a rat model in which nephritis is induced by one-time tail vein injection of doxorubicin.Animals were divided into five groups,namely the naive control group,model control group,Lotensin group,low-dose nephritis Xiaobaifang group and high-dose nephritis Xiaobaifang group.Seven weeks after treatment,we detected rat urine creatinine,serum BUN,Scr,Alb,urineβ2-MG(by ELISA),proteinuria, COL-Ⅳand LN expression in kidney(by immunohistochemical staining), and nephrin mRNA and CD2AP mRNA expression in kidney tissue(by RT-PCR).Results:At the end of 7-week observation,the ratio of proteinuria / urine creatinine values(P<0.05)were reduced in animals from all treatment group with doxorubicin-induced nephritis,and the high-dose nephritis Xiaobaifang treatment had the most significant effects(P<0.05);the urineβ2-MG level(P<0.05)was also decreased in all treatment group,and the high-dose nephritis Xiaobaifang treatment had the most significant effects (P<0.05);the serum albumin concentration(P<0.05)was increased in all treatment group;and high-dose nephritis Xiaobaifang treatment had the most significant effects(P<0.05);the expression of both COL-Ⅳand LN in the kidney were decreased in all treatment group(P<0.05),and the high-dose nephritis Xiaobaifang treatment had the most significant effects (P<0.05);the treatment by nephritis Xiaobaifang did not affect the kidney function in rats with nephritis(P>0.05);the expression of both COL-Ⅳand LN in the kidney tissue were decreased in all treatment group(P<0.05), and the high-dose nephritis Xiaobaifang treatment had the most significant effects(P<0.05);all animals in the treatment group had attenuated foot cell damage,lower nephrin mRNA and CD2AP mRNA expression in kidney tissues,and the high-dose nephritis Xiaobaifang treatment had the most significant effects(P<0.05).Conclusion:nephritis Xiaobaifang can reduce excretion a certain extent, decrease urineβ2-MG level in nephrotic rats and protect tubulo;It can possibly increase serum albumin level by reducing excretion;It can attenuate COL-Ⅳand LN expression in kidney tissues,so it can treat kidney fibrosis;It can improve the foot cell morphology of nephrotic rats, by which it reduced excretion;It can reduce nephrin mRNA and CD2AP mRNA expression in kidney tissues from rats with doxorubicin-induced nephritis,repair injured foot cell,then.reduce excretion.The therapeutic effects with high-dose nephritis Xiaobaifang are better than low-dose nephritis Xiaobaifang or Lotensin.

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