Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

The Expeimental Study on the Influence of Shenshuaixiezhuo Pill on FN、Col-Ⅳ、TGF-β1 of Rats with Renal Interstitial Fribrosis

Author WangLiYan
Tutor ZhangPeiQing
School Heilongjiang University of Traditional Chinese Medicine
Course Chinese medical science
Keywords Shenshuaixiezhuo Pill Renal interstitial fibrosis CollagenⅣ Fibronectin Ttransforming growth factor-β1
CLC R285.5
Type PhD thesis
Year 2008
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Objective:To investigate the effect of the Shenshuaixiezhuo Pill on FN,Col-Ⅳ,TGF-β1 of serum,urine and kidney tissue of renal interstitial fribrosis rats caused by adenine,and to illustrate the mechanism of Shenshuaixiezhuo Pill on delaying renal interstitial fibrosis.Methods:In the experiment,the rats model with renal interstitial fibrosis caused by adenine was adopted.The rats were observed for the change of the common physical order and quantity.The serum,urinary FN, Col-Ⅳ,TGF-β1 in rats were measured by two-layer antibody sandwich enzyme-linked immunosorbent assay(ELISA)method.HE and Masson staining method were used to observe the pathologic change of the experimental rats kidney tissue.The protein expression of FN,Col-Ⅳ, TGF-βand their mRNA in kidney tissue were detected respectively by immunohistochemistry with two steps,Western blot and RT-PCR.Results:The physical status and symptom of the RIF rats were improved obviously after treating by Shenshuaixiezhuo Pill;The urinary FN,Col-Ⅳ,TGF-β1,serum Col-Ⅳwere reduced and the serum FN, TGF-β1 were increased.The protein expression of FN,Col-Ⅳ,TGF-βand their mRNA in kidney tissue were reduced;Compared to comparison group,the crutive effect of Shenshuaixiezhuo Pill was significant difference.Conclusions:Shenshuaixiezhuo Pill could decrease the area of renal interstitial fribrosis through ruducing the inflammation in renal tubular; The serum,urinary FN,Col-Ⅳ,TGF-β1 were changed by reducing they production in kidney;The ECM sedimentation was reduced through depressing the protein expression of FN,Col-Ⅳ,TGF-βand their mRNA.So the renalinterstitial fibrosis was relieved by Shenshuaixiezhuo Pill.

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