The Study on the Influence of Control Factor in Rats with Brain Edema after Acute Intracerebral Hemorrhage Treated by Scalp Penetration Acupuncture
|School||Heilongjiang University of Traditional Chinese Medicine|
|Course||Acupuncture and Massage|
|Keywords||Cerebral edema Scalp penetration acupuncture AQP-4 MMP-2/9|
Objective: To research the influence of control factor in rats with brain edema after acute intracerebral hemorrhage treated by scalp penetration acupuncture.Method: healthy male wistar rats (weight:250-300g) were randomly divided into three groups: A,B and C, each group were randomly divided into 12h、24h、3d、7d four time points, the rats were prepared by rules. The rats in group A were injected 0.9% physiological saline 2ul, the rats in group B and C were injected 0.9% physiological saline with 0.5u/ul collagenase 1ul and 0.9% physiological saline with 7u/ul heparin 1ul, then observe the information and data at assignment time : Neurological deficit scale ,the brain tissue water content, brain tissue patho-form, brain tissue ultramicro- texture and AQP-4,MMP-2,MMP-9 by immunity histochemistry method.Results: 1 .the recordings indicate there is Neurological deficit scale in hemorrhage rats, the peak occurred at 72h and descent then. There are significant disparity between model group and control group.2. The brain tissue water content step up after 24h and the peak occurred at 3d, there are significant disparity between model group and control group.3. The content of EB in brain tissue reached the peak at 24h and keep high level during 24h-72h, there are significant disparity between model group and control group. There are positive correlation between brain tissue water content and the content of EB in brain tissue.4. Light-microscope shows there’s severe pathological change in brain hemorrhagic model group, Nerve cells and colloidal cells swell, and in cell space there’s vacuoles of different sizes and the cell space increases. In treating group, hemorrhage and cell dropsy are distinctly Alleviated.5. Electro-microscope shows compared with normal rats, brain hemorrhagic group shows many changes. Especially in the BBB, mitochondria ranges vague and disappear, mitochondria swell, karyoplasm dilutes compared with normal one , other cell apparatuses are still swelling. But the state in treated group is better.6.The AQP-4 expressed a lot in model group and reach peak at 3d, there are significant disparity between model group and control group (P<0.01) . There are positive correlation between content of AQP-4 and the content of EB in brain tissue.7.There shows MMP-2 expression in model group and reach peak at 24h, there are significant disparity between model group and control group (P<0.01)8. There shows MMP-9 expression in model group and reach peak at 3d, there are significant disparity between model group and control group (P<0.01)Conclusion:1 .The above results shows that the content and changes of AQP-4, MMP-2 and MMP-9 have big relationship with cerebral edema after hemorrhage, they may lead to permeability in BBB and brain tissue water increased.2.The symptom became better after acupuncture , one of the mechanism of anti-edema by acupuncture may be adjust the expression of AQP-4,MMP-2 and MMP-9, diminished break in BBB, degrade brain water content, improved symptom accompanied with cerebral edema.