The Adhesion and Antagonistic Effects, and Fermentation Characteristics of Bifidobacterium Bifidum S17

Adhesion and colonization of bifidobacteria to intestinal epithelial cells is consideredimportant to choose Bifidobacterium strains, it is related to bifidobacteria whether to play theecological effects. Bifidobacterium colonization in the intestinal epithelial cells, the first thingis adhesion. If only as the passing bacteria but not colonization in the intestine, it can not fullplay the physiological functions of bifidobacteria. In human intestinal tract, bifidobacteria caninhibitory variety of enteric pathogens. Bifidobacteria can produce extracellular enzymes ableto reduce specific sites of pathogens or their toxins. After colonization of bifidobacteria in theintestinal epithelial cells, it can form a layer of bioflim to inhibit the pathogen adhesion tointestinal mucosa. Bifidobacteria can produce bacteriocins, antibiotics, hydrogen peroxide andother substances to stimulate the body to produce catalase inhibition and killingGram-negative bacteria. In this study, we use the Hela cell line as model and Bifidobacteriumlongum NCC2705as the control, to study the adhesion of Bifidobacterium bifidum S17onHela cells, bifidobacteria on the antagonistic effects of several common enteric pathogens,Bifidobacterium bifidum S17and Streptococcus thermophilus and Lactobacillus delbrueckiiBulgaria subspecies mixed fermented yoghurt process conditions optimization. Providetheoretical basis and guidance for the development of Bifidobacterium probiotics. The mainresults obtained are as follows.(1) Bifidobacterium bifidum S17and Bifidobacterium longum NCC2705can strongadhesion on Hela cells. Bifidobacterium bifidum S17in Hela cells, the adhesion index was19.42±5.43; Bifidobacterium longum NCC2705in Hela cells, the adhesion index was1.91±1.19. Bifidobacterium bifidum S17adhesion on Hela cells was stronger thanBifidobacterium longum NCC2705.(2) Bifidobacterium bifidum S17and Bifidobacterium longum NCC2705were significantantagonism to Shigella301, Enterococcus faecalis V583, pathogenic E. coli EPEC, andSalmonella. Bifidobacterium bifidum S17antagonistic ability to enteric pathogens is strongerthan Bifidobacterium longum NCC2705.(3) Bifidobacterium bifidum S17and Bifidobacterium longum NCC2705culture withfour enteric pathogens can reduce the intracellular and secreted into the supernatant of TNF-α,IL-1β, and IL-8levels. Bifidobacterium bifidum S17has a certain anti-inflammatory capacity, it can stimulate the intestinal immune system. The anti-inflammatory ability ofBifidobacterium bifidum S17is stronger than Bifidobacterium longum NCC2705.(4) Optimization of process parameters for the Bifidobacterium bifidum S17fermentedmilk was fermentation time of6h, inoculum was10%, Bifidobacterium bifidum S17andmixed fermentation agent ratio of2:1, the fermentation temperature of42℃. Afterfermentation, the sensory score was97points, acidity was94.1oT. Bifidobacterium bifidumS17fermented milk, the acidity increases and the number of viable cells is reduced with theextension of the cold storage time. The changes are within the scope of national standards,and do not affect the consumption and sale of fermented milk.