Preparation and Preservation of Bilayer Lipid Membrane and the Interaction with Gemini Surfactant
|Course||Physical and chemical|
|Keywords||Bilayer lipid membranes Lecithin Cholesterol Preparation Optimization Stability Gemini Surfactants Synthesis Cryopreservation Cyclic voltammetry|
Biofilm plays a very important role in the process of life, to recognize that the phenomenon of life, we must first understand the process in biofilms. However, since the composition of the biofilm, the complexity of the structure, function, carried out in situ on-site observation and research is very difficult, therefore, prepared in the laboratory of the analog system is the basis of the various studies and premise. Bilayer lipid membranes as a biofilm model system, both with biofilm basic framework, but also overcome the complexity of the biofilm itself, is an excellent model to study biofilm system. In recent years, artificial the simulated bilayer lipid membranes as a biofilm model has been extensive attention, showing the interaction between applications in the testing of environmental pollutants, life detection of active substances, drugs and biofilm broad prospects for development. For artificial simulation of bilayer lipid membranes presence of membrane stability is relatively poor, it is difficult to meet the needs of scientific experiments and practical applications, taking into account the importance of the bilayer lipid membrane matrix membrane biochemical changes This thesis is to explore to improve the bilayer lipid membrane stability and a long time to save the bilayer lipid membranes: synthesis of novel Gemini surfactant agents bilayer lipid membrane metal support substrate by electrochemical means Gemini surfactant bilayer lipid membrane interactions in order to provide the basis for the study of drugs and biofilm interactions. The main conclusions of this thesis is as follows: (1) to tetradecyl bromide and tetramethyl ethylenediamine as raw material, through nucleophilic addition to salt synthesis coupled cation type the dibrominated-N N-bis (dimethyl alkyl) ethylene diammonium (C 14 -2-C 14 · 2Br - sup>) Quaternary Ammonium Surfactants The active agent, is purified by recrystallization with ethyl acetate / ethanol to give Gemini surfactant samples. Infrared spectroscopy, nuclear magnetic resonance spectroscopy studies have shown that The Gemini surfactant prepared by this method for the target molecule [C 14 -2-C the 14 · 2Br - < The n-decane solution (2) the use of lecithin as a film-forming liquid, in the end surface of the stainless steel wire can be prepared a stable bilayer lipid membranes; cholesterol added to the film-forming solution can improve the film-forming efficiency and the stability of the film. Metallographic sandpaper end surface of the stainless steel wire, can expand the area of ??film formation of bilayer lipid membranes. (3) gradual freezing method can greatly improve the stable bilayer lipid membranes, and can greatly reduce the impact of external shocks and other factors on the bilayer lipid membranes. (4) surfactant tetradecyl trimethyl ammonium bromide and Gemini surface active agent C 14 -2-C 14 · 2Br - sup>, will change the structure of the lecithin bilayer lipid membranes and cholesterol lecithin bilayer lipid membranes. , Tetradecyl trimethyl ammonium bromide in the range of concentration of 0.0004% -0.015% of the film resistor is gradually reduced, the membrane capacitance gradually increases to the final film resistors, membrane capacitance tends to a constant value; at a concentration of 0.0004 % -0.015% range, Gemini surfactant C 14 -2-C 14 · 2Br - sup> of lecithin bilayer lipid the film is similar to the impact and tetradecyl trimethyl ammonium bromide, but the influence of the bilayer lipid membrane to add cholesterol, not only does the film resistor is reduced, but the film resistance increasing membrane capacitance increases or decreases the change is relatively complex. The final membrane resistance and membrane capacitance tends to a constant value.