Construction of Bioelectrocatalytical System Based on Lipidic Cubic Phase and Applications in Bioelectronic Devices
|School||Anhui Normal University|
|Keywords||Lipid cubic liquid crystal phase Electrochemical catalytic Bioelectrocatalysis Electrochemical Biosensors A biofuel cell|
Lipidic cubic liquid crystal phase is self-assemble into a closed lipid bilayer containing a bicontinuous watercourse structure by a certain concentration of the amphiphilic substance in the aqueous phase and the lipid bilayer and then twisted into a three-dimensional class biofilm properties The three-dimensional structure. The lipidic cubic liquid crystal phase having a large viscosity, good biocompatibility characteristics, can be stable in the aqueous phase. These features make the lipidic cubic liquid crystal phase can be used as the embedding matrix biocatalyst (enzyme or protein), create bioelectrocatalytic system, and then construct the bio-electronic devices (biosensors, fuel cells, etc.). The paper select monooleate (monoolein, MO) Preparation of lipid cubic liquid crystal, and as a the embedding protein (hemoglobin), the substrate of the enzyme (alcohol dehydrogenase, laccase), to build a bio-electronic devices. Specifically summarized as follows: 1 Preparation of a the lipidic cubic liquid crystal embedded redox protein, hemoglobin (Hemoglobin, Hb) modified electrode, examine the behavior of the direct electron transfer between the hemoglobin and the electrode, and then construct the third generation of hydrogen peroxide biosensors. The results show that the hemoglobin inter-electrode electron transfer kinetic constants (Ks) of 3.03 s-1, the catalytic substrate hydrogen peroxide, the apparent Michaelis constant (ΚaMpp) for 0.27 mM, constructed by the third generation of hydrogen peroxide sensor The linear range of 7 to 239μM detection limit of 3.1μM. The prepared lipid cubic liquid crystal phase the embedded tetrachlorobenquinone (Tetrachlorobenzoquinone, TCBQ) carbon nanotube modified electrode (MO-TCBQ/SWNT/GC) electrocatalytic properties of the electrode of NADH NADH detection The linear range of 5 to 1650μM detection limit of 0.82μM. Further prepared TCBQ and alcohol dehydrogenase (Alcohol Dehydrogenase ADH) the total embedded in the lipid cubic liquid crystal phase biological electrode (MO-TCBQ-ADH/SWNT/GC), and then construct the ethanol dehydrogenase-based biosensor the biological electrode for ethanol detection linear range of 0.2 to 13 mM, a detection limit of 0.05 mM. Prepared toluidine blue (Toluidine blue, TB) and alcohol dehydrogenase (ADH), embedded in the the lipidic cubic liquid crystal phase modified electrode (MO-TB-ADH/GC), based on the electrode ethanol The Catalytic Oxidation constructed the anode of the biofuel cell. Prepared laccase (Laccase) the modified electrode (MO-Laccase/GC) embedded in the lipid cubic liquid crystal phase, the ABTS solution based on the electrode in good catalytic oxygen reduction performance, build the cathode of the battery. Based on this further build based lipidic cubic liquid crystal phase diaphragm ethanol / oxygen biofuel cell. The study showed that under the conditions of room temperature and oxygen saturation, the battery open circuit potential of 0.38 V, the operating voltage of 0.06 V, the output power can be achieved of 0.2μW/cm to 2.