Biological Effects of Biomass Sand Stabilization Material Prepared with Lignosulfonate
|School||Nanjing Forestry University|
|Keywords||Biomass sand stabilization material Biodegradable White rot fungi Laccase -ABTS system Manganese peroxidase|
Biomass sand stabilization material (Biomass sand stabilization material, BSSM) Nanjing Forestry University grasses lignin pulping liquor is the main raw material, and sand-fixation and ecological restoration in one new sand fixation functional polymer materials. Thesis paper mill byproducts - ammonium lignosulfonate synthesized BSSM is clear the environmental behavior of the BSSM application process, especially biodegradable performance and exchage BSSM on the choice of the cabbage species and Mao sheep grass seed, plant The growth impact test, and the selection of white rot fungi, laccase-ABTS system, manganese peroxidase BSSM test. Choice of the sheep grass seed of cabbage species and Mao, the BSSM of plant growth affect the test results showed that the germination rate of cabbage and high-Mao sheep: BSSM (200-800 ppm) had no significant effect: but the BSSM can promote cabbage seeds and Mao sheep grass seed germination, shorten the time of seed germination, and promote the accumulation of biomass. Select the white-rot fungus to BSSM through 36 days of culture, BSSM lignosulfonate decreased 16.3% BSSM may occur degradation, while the the BSSM organic matter content in almost no change, to some extent, in line with humus formation doctrine. White rot fungus Phanerochaete sordiada YK-624 strain the Kirk culture medium preparation and the beginning of the activity of the enzyme solution containing higher manganese peroxidase (MnP) purified enzyme play better activity conditions: temperature 35 ℃ , H2O20.6 mmol / L, pH value of 4.5, the concentration of Mn2 less influence. Prepared manganese peroxidase with lignin degradation capacity, with BSSM pilot study, the research shows that: BSSM did not inhibit the enzyme activity of MnP has better biodegradability, and initially identified BSSM After after MnP processing, the presence of certain demethoxylation phenomenon, the destruction of a benzene ring skeleton, its structure unsaturated enhanced quinones may generate the intermediate product of the unsaturated structure. In the laccase enzymatic properties are measured on the basis of determined laccase-ABTS system of BSSM biological degradation conditions: and 1mmol / ABTS.pH 4.5 200 mmol / L acetic acid - sodium acetate buffer system, the reaction temperature is 35 ° C. And then by measuring the laccase activity in the course of the reaction as well as a sulfonic acid group, a conjugated carbonyl group, quinone structure content changes, combined with UV - visible spectroscopy, infrared spectroscopy, hydrogen - nuclear magnetic resonance spectrum, and the molecular weight distribution analysis, confirmed BSSM no inhibition on the laccase activity, having a good biological degradation properties, and preliminarily determines the BSSM reaction sites in the presence of laccase-ABTS system in biodegradation is mainly a phenolic hydroxyl group, a carbonyl group, a methoxy group, a benzene ring side chain substituent, etc., the quinone is an important intermediate product. BSSM high molecular weight fraction decreased, especially between 8000-18300 reduce the amount is significant, and part was an increase in molecular weight of less than 8000. Preliminary laccase-ABTS system degradation and aggregation of the dual role of BSSM.