Phylogenetic Relationship Analysis Based on RAPD among Partial Species and Cultivars of Paeonia L.
|School||Henan University of Science and Technology|
|Keywords||Paeonia L. Phylogenetic relationship RAPD marker Species Cultivar|
Paeonia is the only genus in Paeoniaceae which contains section Moutan, sectionOnaepia and section Paeoinia. China is the center of the origin, evolution, anddiversity of Paeoniaceae, especially the section Moutan is unique in China. Based onRAPD technique, to carry out the genetic relationship among partial species andcultivars of Paeonia is helpful to investigate the taxonomic status of Paeonia and willprovide a molecular basis for the breeding of new varieties.In this study, five factors which affect the single-primer RAPD PCR amplificationresult for Paeonia germplasms were optimized. The single-primer RAPD amplificationsystem for Paeonia germplasms were established, which contains1.75mmol/L Mg2+,0.2mmol/L dNTPs,1.5U Taq DNA polymerase,0.5μmol/L primer, and18ng DNA.In addition, the double-primers RAPD amplification system for Paeonia germplasmswas also established, which contains1.5mmol/L Mg2+,0.3mmol/L dNTPs,1.5U TaqDNA polymerase,0.4μmol/L each primer, and12ng DNA. Optimized single primeror double primers RAPD amplification system were stable enough, which can be usedto analyze the phylogenetic relationship of Paeonia L. germplasms.Based on the established single-primer and double-primers RAPD amplificationsystem,10single primers and two pair of double primers were screened forconstruction of the phylogenetic relationship of Paeonia germplasms. The totalnumbers of the RAPD bands were108among the46germplasm. Tree peony speciesamplified81bands, of which75bands are polymorphic loci, and the polymorphic ratewas up to92.5%. Tree peony cultivars amplified99bands, including76polymorphicloci, and the polymorphic rate was76.7%. The herbaceous peony cultivars amplified79bands, including55polymorphic loci, and the polymorphic rate was69.6%.Based on NTSYS-pc2.10statistical analysis software, the phylogeneticrelationships of46germplasms of Paeonia were analyzed with UPGMA method. Themain conclusions are as follows. Firstly, when the genetic distance was0.9,11 herbaceous peony cultivars cluster to a group, and35tree peony （6wild species,17cultivars from cultivar group of tree peony from central plains of China, and12treepeony cultivars from aboard） cluster to another group. Secondly, the phylogeneticrelationship of6wild spcies of tree peony showed that the leathery disk groups such asP. decomposita, P. qiui and P. rockii cluster to a group, and fleshy disk groups such asP. potaninii, P. lutea and P. delavayi cluster to another group. Thirdly, the phylogeneticrelationships of wild species of tree peony to tree peony cultivars from far to near are P.delavayi, P. lutea, P. potaninii, P. decomposita, P. rockii, and P. qiui. Forthly, Theclustering results of the cultivar group of tree peony from central plains of China withtree peony cultivar from aboard show that the cultivars which come from neighboringarea have higher genetic similarity. Fifthly, the ‘Itoy Hybrid’, interspecific hybrid, ismore similar with tree peony, which has closer relation to tree peony cv.‘Jinge’.Sixthly, herbaceous peony cv.‘Xiangyangqihua’ had far relationship with the other10cultivars. Seventhly, the herbaceous peony cultivars which have similar color mayhave closer phylogenetic relationship.