Study on Pathogenesis and Dissolution in Vitro of Canine Uroliths
|Course||Clinical Veterinary Medicine|
|Keywords||canine uroliths component analysis of canine uroliths vitro dissolution of stones liver diet|
Urolithiasis is one of the most frequent urinary system diseases of canine. Though abundant of researches were done on the pathogenesis both home and abroad, no certain conclusion has been gotten until now. In order to get the pathogenesis, lots of researches and analysis have been done in human and veterinary medicine, and kinds of high technology analysis tools have been used in uroliths study. Reasonable selection and combined use of several analytical methods are necessary to define the component and construction of uroliths, what’s more, it can provide more information on the pathogenesis of urolithiasis.Diet played an important role in the formation of urinary sediment and supersaturation urine. There are a lot of clinical surveys showed that persistently feeding of animal liver was apt to form uroliths, but it still lacked of animal experiment to certificate. In order to clarify the relation between liver diet and urolithiasis, experimental dogs were fed with pork liver and the concentration of several blood and urine indicators were detected during the experiment.Surgery the most principal treatment for canine urolithiasis at present can’t deal with the prevention and recurrence of this desease. As a treatment which may get rid of urine sediment and small calculi, moreover reduce the use of surgery and the recurrence of calculi, litholytic therapy should deserve more attention. In the study, three kinds of solutions were used in the vitro dissolution experiment, which not only aim to compare the dissolution effect but also provide more information for clinical application.Test I Component analysis of canine uroliths. Chemical analysis, Fourier transform infrared spectroscopy, X-ray energy dispersive spectrometer(EDS), and scanning electron microscope(SEM) were used to analyse39uroliths samples. The results revealed that the major component of4samples(10.3%) was the calcium oxalate,9(23.1%)were urate calculi,26(66.6%) were struvite and calcium phosphate mixed stones.Test II The effect of liver diet on the change of canine blood and urine indicators. In the study, twelve healthy dogs were randomly divided into two groups, one was the control group which was fed with normal food, the other was the experimental group which was fed with pork liver. During8weeks of experiment, blood and24hours urine samples were collected from the dogs once a week. Blood serums were detected for the concentration of sodium, potassium, chlorine, calcium, magnesium, phosphorus, creatinine, urate and urea nitrogen, while the urine samples were detected for urine volume, urine pH, and the concentration of sodium, potassium, chlorine, calcium, magnesium, phosphorus, ammonium, creatinine and urate. All the results were analysed by T test with SPSS sofeware. Compared with the control group, the concentration of phosphorus, magnesium and ammonium in urine increased significantly, while sodium decreased significantly; the concentration of urea nitrogen in blood serum increased significantly, while calcium and creatinine decreased significantly. The results showed that liver diet can lead to stone formation urine and promote the formation of struvite crystals.Test III Phosphatic calculi dissolution in vitro. Renacidin, Suby’G and Linshixiao (self-developed) were used to dissolve the phsphatic calculi samples in vitro. Each solution was divided into four groups, respectively representing pH4,4.5,5,5.5. On the condition of pH5, the stone weight decreased rate of Renacidin was significantly lower than Suby’G and Linshixiao, while Linshixiao was significantly higher than Renacidin and Suby’G on pH5.5. The dissolution rate of Renacidin was significantly lower than Suby’G and Linshixiao on pH5.5. The dissolution effect of the three solutions had no difference on other pH. Lingshixiao caused the least injured to the bladder mucosa compared with Renacidin and Suby’G.