Dissertation
Dissertation > Medicine, health > Chinese Medicine > TCM Internal Medicine > Modern medicine, internal diseases

Study on the Mechanism by Which Traditional Chinese Medicine Bear Bile Formulation Treats Nonalcoholic Steatohepatitis Based on NF-κB Pathway

Author LiYing
Tutor HongZhenFeng
School Fujian University of Traditional Chinese Medicine
Course Chinese and Western medicine combined with the basis
Keywords Nonalcoholic steatohepatitis bear bile formulation traditional Chinesemedicine (TCM) inflammatory NF-κB
CLC R259
Type Master's thesis
Year 2013
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Objective:This study was designed to evaluate the therapeutic effect of traditional Chinese medicine Bear Bile Formulation on Nonalcoholic steatohepatitis (NASH). To investigate the molecular anti-inflammatory mechanism of Bear Bile Formulation by observing its influence on the expression of key regulatory factors in the NF-κB inflammatory pathway, such as NF-κB, TNF-α, etc. The aim of this study was to provide a theoretical basis for the clinical application of bear bile TCM.Method:In the thesis,60adult male SD rats were randomly divided into blank group, model group, compound methionine and choline bitartrate group, bear bile TCM low dose group, bear bile TCM middle dose group and bear bile TCM high dose group. Model group and each treatment group were fed with high fat diet and injected with CC14to generate NASH model. Meanwhile, the compound methionine and choline bitartrate group were orally treated with compound methionine and choline bitartrate at a dose of0.24g/kg/d, the bear bile TCM group were orally treated with bear bile TCM at a dose of0.462g/kg/d,0.924g/kg/d and1.848g/kg/d respectively, while the blank group and model group were orally treated with saline respectively. After30days’treatment, rats of each group were weighted and sacrificed and the liver tissues were collected to evaluate the liver weight (LW) and liver index (LI). The serological changes were observed by ALT, AST, TG, TC, HDL-C, LDL-C. The histological changes of liver were observed by HE staining. Then the expression of NF-κB, TNF-α, IL-6, COX-2in liver tissues were determined by RT-PCR and Immunohistochemistry(IHC). Result:(1) It was showed that bear bile TCM group significantly reduced body weight(BW) and LI and ALT, AST compared with that of Model group in NASH rats which had dose dependent(P<0.05).(2) It was showed that bear bile TCM group significantly reduced TG, TC, HDL-C, LDL-C compared with that of Model group in NASH rats which had dose dependent(P<0.05).(3)HE staining showed that there were significant fatty inflammatory changes in the liver tissue of model group, and the changes of compound methionine and choline bitartrate and bear bile TCM group were significantly reduced compared to the model group which had dose dependent.(4) The mRNA and protein expression of NF-κB, TNF-α, COX-2, IL-6in compound methionine and choline bitartrate group and bear bile TCM group were significantly decreased, compared with that in the model group which has dose dependent(P<0.05).Conclusion:(1) Bear bile TCM could significantly improve BW, LI, ALT and AST of the rat model, which suggested that the bear bile TCM had a significant dose-dependent protective effect on NASH rats.(2) Bear bile TCM could significantly improve TG, TC, HDL-C and LDL-C of the rat model, which suggested that the bear bile TCM had a significant dose-dependent effect of lipid metabolism regulation on NASH rats.(3) Bear bile TCM could significantly improve the fatty inflammatory in the liver tissue, which suggested that the bear bile TCM had a significant dose-dependent effect on NASH rats..(4) Bear bile TCM could significantly reduce the expression of NF-κB, TNF-α, IL-6and COX-2of the rat model, which suggested that the bear bile TCM had a significant dose-dependent anti-inflammatory effect on NASH rats.

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