Dissertation
Dissertation > Medicine, health > Chinese Medicine > Chinese Minority Medicine

Antioxidant and Antidiabetic Activities of Garcinia Xanthochymus

Author FuMeng
Tutor YangGuangZhong
School Central South University for Nationalities
Course Medicinal Chemistry
Keywords Garcinia xanthochymu Antioxidant activity Antidiabetic activity Xanthones Biological activity
CLC R29
Type Master's thesis
Year 2012
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Garcinia xanthochymu is a perennial medicinal plant native to the south andsouthwest of Yunnan of China which can grow up to10-20m. The fruit of G.xanthochymus has been used widely as a traditional folk medicine for bilious condition,diarrhea, and dysentery. Previous phytochemical investigations on G. xanthochymusresulted in the isolation of benzophenones, flavonoids, triterpenes and xanthones fromthe wood, fresh leaves and fruits. Xanthones as the active ingredient have widelybiological activities, such as antioxidant activity, antidiabetic activity, antibacterial, anti-HIV virus and so on. The petroleum ether (PFr.), ethyl acetate (EFr.), n-butanol (BFr.)and water fraction (WFr.) from leaf, root, fruit of G. xanthochymus were obtained bysystem solvent extraction, and the antioxidant and antidiabetic activity were assessed byusing appropriate assay systems.The antioxidant activity of PFr., EFr., BFr. and WFr. from each plant part of G.xanthochymus was assessed by determining the total phenolic content, evaluating thereducing power, measuring the capability for scavenging free radicals, as well as itsability to inhibit DNA cleavage. Six fractions showed antioxidant activity, especially theEFr. from the leaf of G. xanthochymus (DPPH IC50=6.10±0.01μg/ml, ABTS IC50=6.74±0.09μg/ml) showed a potent antioxidant activity which was similar to that ofthe standard antioxidant BHT (DPPH IC50=5.67±0.02μg/ml, ABTS IC50=9.29±0.14μg/ml). This study provided a theory reference for the further isolation of naturalantioxidants from G. xanthochymus.To evaluate the antidiabetic potential of G. xanthochymus, the α-glucosidase andα-amylase inhibitory activities of PFr., EFr., BFr. and WFr. from root, leaf and fruitwere assessed. Seven fractions from G. xanthochymus had a dose-dependent inhibitoryactivity. The root EFr. and BFr. showed the highest α-glucosidase inhibitory activity(IC50=13.01±0.38μg/ml,14.05±0.24μg/ml). Root PFr., EFr. and BFr. showed thehighest α-amylase inhibitory activity (IC50=0.482±0.079mg/ml,0.667±0.071mg/ml,0.831±0.062mg/ml). Five extracts (leaf-WFr., leaf-PFr., root-WFr., fruit-BFr. andfruit-WFr.) showed no α-glucosidase or α-amylase inhibitory activities. In HepG2glucose consumption experiments, fruit-EFr. could remarkably promote the cellsconsume glucose in7.5-30mg/ml (P <0.001) with a dose-dependency. leaf-EFr.,root-BFr. and root-EFr. also showed promoting glucose consumption activity, and theGC rate reached3.08,3.12,1.93in the concentration of30mg/ml,25mg/ml,7.5mg/ml. Diabetes mellitus in mice was induced by streptozotocin–nicotinamide. After10days oftreatment with glibenclamide, leaf-EFr. and fruit-EFr., the blood glucose excursion wassignificantly lower (P <0.05) than that of the diabetic control. It showed that G.xanthochymus had potential diabetes activity, which provide a basic theory for thefurther biological development.

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