Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Separation and extraction of active ingredients

Separation and Antioxidant Activity of the Chemical Constituents from Eupatorium Fortuneiturcz. and Amomum Longiligulare T.L.Wu.

Author LiuJinPeng
Tutor DuZuoZhen; YeYang
School Zhejiang Technology and Business University
Course Food Science and Engineering
Keywords Eupatorium fortunei Turcz. Amomum longiligulareT.L.Wu. chemical constituments antioxidative activity
CLC R284.2
Type Master's thesis
Year 2013
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Eupatorium fortunei Turcz. and Amomum longiligulare T.L.Wu. are two plant species respectively from the genus Eupatorium L. of the family Compositae and Amomum L. of the family Zingiberaceae. We carried a systematical phytochemistry investigation on the two plant species. A total of20compounds were isolated by means of a variety of isolating technologies, such as column chromatography over silica gel, MCI, Sephadex LH-20, preparative HPLC, etc. and identified through combinational analyses of spectroscopic data. The structures of these compounds mainly involved thymol, sesquiterpene, sterol, phenolic acid, diarylheptanoid and flavonol.Studies on the chemical constituents of E. fortunei led to6compounds,9-hydroxythymol (PL1),8,9-dihydroxythymol (PL2), 8-hydroxythymol (PL3), clovane-2β,9a-diol (PL4), caryolane-1β,9β-diol (PL5), stigmasta-7,22-dien-3a-ol (PL6). Compound PL3was the first time to be isolated from the plant species.Studies on the chemical constituents of A. longiligulare led to14compounds,4-(4-hydroxyphenyl)-2-butanone (SRI),4-methoxybenzoic acid (SR2),3,5-diacetoxy-1,7-bis(3,4-dihydroxyphenyl)heptane (SR3),1,7-bis(4-hydroxyphenyl)-5-hepten-3-one (SR4),1,7-bis(4-hydroxyphenyl)-3-heptanone (SR5),3-hydroxy-1,7-bis(4-hydroxyphenyl)heptane (SR6),1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)-5-hepten-3-one (SR7),3,5-dihydroxy-l-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)heptane (SR8),3,5-dihydroxy-1-(3,4-dihydroxyphenyl)-7-(4-hydroxy-3-methoxyphenyl) heptane (SR9),1,5-epoxy-1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)heptane (SR10),3,5,7-trihydroxy-4’-methoxyflavone (SR11),3,5-dihydroxy-7,4’-dimethoxyflavone (SR12),3,5,3’-trihydroxy-7,4’-dimethoxy flavone (SR13), sitosterol(SR14). SRI-SRI4was the first time to be isolated from the plant species.SR3-SR13belong to diphenylheptanes and flavonoids were applied for the preliminary evaluation of their antioxidative activities by scavenging free radical DPPH. The results showed that the diphenylheptanes compounds showed stronger antioxidant activity than the flavonols. Among the diphenylheptanes, the compounds with pyrocatechol and alkylhydroxy exhibited the strongest DPPH scavenging activity, the compounds only with pyrocatechol or alkylhydroxy exhibited the stronger activity and the compounds without pyrocatechol and alkylhydroxy exhibited the weakest activity. which indicated that pyrocatechol and alkylhydroxy were effective antioxidant activity groups of these compounds.In summary, the present investigation on the chemical constitutes of E. fortunei and A. longiligulare,20compounds were isolated and structurally elucidated. Our results enlarge the knowledge of the chemical base of these herbs.

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