Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification

Study on Bioactive Components from the Twigs of Aglaia Odorata Var.Microphyllina

Author LiuShuai
Tutor WangJian; DaiHaoFu
School Hainan University
Course NaYao learn
Keywords Aglaia odorata var. microphyllina chemical constituents structure identification cytotoxic activity antibacterial activity
CLC R284.1
Type Master's thesis
Year 2013
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Aglaia odorata var. microphyllina belongs to the genus Aglaia of family Meliaceae. It is a common decorative plant, which has also been used as a traditional medicine for traumatic injury by the Li folk people in Hainan. Previous chemical investigations on this genus have led to the isolations of a series of structurally interesting compounds such as flavaglines, bisamides, triterpenoids, diterpenoids and sesquiterpenoids, some of which showed various bioactivities, such as insecticidal, cytotoxic, and antibacterial activities. A. odorata var. microphyllina is a shrub endemic to Hainan province. To search for bioactive constituents with medicinal value from the twigs of A. odorata var. microphyllina, chemical constituents and bioactivities were studied under cytotoxic and antibacterial bioassay guidance.Twenty five compounds were isolated from the ethanol extract of the twigs of A. odorata var. microphyllina by various column chromatography, and their structures were indentified by physicochemical properties and spectral data (UV, IR, MS,1D and2D NMR), including:8b-methoxy-desmethylrocaglamide (1),3’-hydroxy-8b-methoxy-rocaglamide (2), desmethylrocaglamide (3),3’-hydroxy-desmethylrocaglamide (4),3’-hydroxy-8b-methoxy-marikarin (5),4α,10β-dihydroxy-1,5-trans-guai-6(7)-en-11-one (6),1α,4α,7α-trihydroxy-eudesman-11(12)-ene (7),1α,6β,12-trihydroxy-1,5-trans-guai-6(7)-ene (8),4α,7β,11-trihydroxy-1,5-trans-guai-10(14)-ene (9),4α,10α,11-trihydroxy-1,5-trans-guai-7(8)-ene (10), guaianediol (11), orientalol A (12), orientalol B (13),1β,6β-dihydroxy-eudesman-4-one (14),4(15)-eudesmen-1β,7,11-triol (15),4α,10β,11-trihydroxy-1,5-trans-guai-6-ene (16),6,15α-epoxy-1β,4β-dihydroxy-eudesmane (17),2-hydroxy-butandioic acid1-methylester (18), jacarreubin (19),11α,12βH-dolabella-4,8(17)-dien-3α,7β,18-triol (20),9-methoxy-7’,8’-cis-7",8"-cis-buddlenol B (21),(+)-diasyringaresinol (22), N-methyl-5-hydroxy-Δ3-pyrrolin-2-one (23), marmin (24), and daucosterol (25), respectively. Compounds1~25were isolated from A. odorata var. microphyllina for the first time. Among them,9new compounds were isolated, including compounds1~2(two new rocaglamides), compounds6~10(five new sesquiterpenoids), compound20(a new diterpenoid) and compound21(a new sesquilignan). The cytotoxic activities of compounds1~25were tested by MTT method and compounds3,4,5,6,8,11, and20showed inhibitory activities against tumor cells. The inhibitory activities against Staphylococcus aureus of all the compounds were measured by paper disco diffusion method, and among them, compounds18,19, and20exhibited antibacterial activities.

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