Dissertation
Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Effects of Salidroside on Myocardial Acute Exhaustive Exercise Injury Through MAPK Pathway

Author WangYunRu
Tutor CaoXueBin
School Hebei Medical University
Course Internal Medicine
Keywords Salidroside exhaustive exercise myocardial damage stress MAPK
CLC R285.5
Type Master's thesis
Year 2013
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Objective:Right amount of movement is beneficial to improving thephysique level, reducing the pressure, preventing and controlling diseases.Conversely one-off exhausting or long-term excessive activities will makehuman organisms decline and further impair their physiological capacity,causing multiple organ impairment. In recent years, many successive sportssudden death in all kinds of sports occasions have been reported by domesticand foreign media. The incidence of the cardiogenic sudden death has a highrate of the sport injury. It has become the focus of increasing concern. A lot ofresearch shows that Salidroside has effective protection on myocardial acuteexhaustive exercise injury.Salidroside is the effective ingredient of the traditional Chinese herb,alarge number of studies in recent years show that It has the special function ofanti-fatigue, anti-anoxic, anti-tumor, anti-oxidative, regulatinging immune,improving mental ability, enhancing strength, preventing arteriosclerosis,resisting radialization, resisting mutation.The effects of Salidroside on thecardiovascular system are manifold. The recent studies have proved that it iseffective in enhancing myocardial con-tractility, protecting the hearts againstmyocardial ischemia and reperfusion injury and the hydrogen peroxide injury.Mitogen-Activated Protein Kinase, MAPK is one of important signaltransduction pathway in organisms. Currently, the domestic studies aboutMAPK mainly concentrate on the myocardial ischemia and myocardialischemia-reperfusion injury. However, the research on acute exhaustiveexercise injury is relatively rare.In this experiment, to explore the protective action of Salidroside againstexhaustive exercise induced myocardial damage in rats. explore its mechanismof effect by the animal models of acute exhaustive exercise injury. The expression of MAPK in cardiac tissue is assayed by western blot. investigatethe expression and significance of P-ERK and p-P38protein in exhaustive,exercise induced myocardial damage. ELISA study for concentration of CK、CK-MB、LDH、SOD、 MDA in plasma and myocardial tissue. Relevantmechanism will certain provide us with a new method in treating andpreventing heart disease diseases.Methods:1Healthy SD40male rats were randomly divided into four groups(n=10): control group, acute exhaustived group, salidroside group, salidrosideand acute exhaustived group.2The control and Acute exhaustive group were given0.9%Nacl(12ml·kg-1·d-1) for14consecutive days. The salidroside group and salidrosideand acute exhaustived group were given salidroside (24mg·kg-1·d-1)for14consecutive days. Acute exhaustive rat model were prepared by exhaustiveswimming in pool.3ELISA study for concentration of CK、CK-MB、LDH、SOD、 MDAin plasma and myocardial tissue.4Western blot was used to detect the expression of ERK、p-ERK、P38and p-P38protein.Results:Compared with control group, level of CK、CK-MB、LDH in the bloodserum of the acute exhaustived group were extremely higher after one-timeexhaustive swimming (P<0.05).There were no significant difference betweensalidroside group and control group (P>0.05). Compared with acuteexhaustived group, the level of CK、 LDH decreased significantly insalidroside and acute exhaustived group (P<0.05). The level of CK-MBreduced in salidroside and acute exhaustived group (P<0.05).Compared with control group, the contents of MDA in the myocardialtissue of the acute exhaustived group significantly increased(P<0.05),whilethere was no difference between salidroside group and control group (P>0.05). Salidroside and acute exhaustived group has a lower MDA level than those in the acute exhaustived group(P<0.05).Compared with control group, SOD activity in the myocardial tissue ofthe acute exhaustived group significantly decreased(P<0.05),salidroside cansignificantly strengthen SOD activity after one-time exhaustive swimming(P<0.05), but there was no significant differences between salidroside groupand control group (P>0.05).Compared with control group, the Phospho-ERK in the acuteexhaustived group were more reinforcement (P<0.05). salidroside cansignificantly increase the phosphorylation level of ERK in the model ofexhaustive swimming of rats (P<0.05). there was no significant differencesbetween salidroside group and control group (P>0.05).Compared with control group, the Phospho-p38in the acute exhaustivedgroup were more reinforcement (P<0.05). salidroside can significantly declinethe phosphorylation level of p38in the model of exhaustive swimming of rats(P<0.05). there was no significant differences between salidroside group andcontrol group (P>0.05).Conclusion:1Salidroside can protect myocardium from exhaustive exercise induceddamage.2Salidroside protect myocardium and against exercise induced damage.Its mechanism may be involved in increasing antioxidation capability,activating the phosphorylation level of ERK and p38in MAPK signalingpathway, inhibiting of cell apoptosiss and so on.

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