Dissertation > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology

Gualou Xiebai Banxia Decoction pretreatment on myocardial ischemia reperfusion to investigate the effects and mechanism of apoptosis of myocardial cell injury in rats

Author JinHongBin
Tutor ZhangBingTian
School Hunan University of Traditional Chinese Medicine
Course Clinical basis for TCM
Keywords Gualou Xiebai Banxia Decoction Ischemia myocardial Reperfusion injury Apoptosis
CLC R285.5
Type Master's thesis
Year 2012
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Objective:The study Gualou Xiebai Banxia Decoction myocard ial reperfusion injury induced apoptosis, and to explore it s possible mechanism.Methods:Left anterior descending coronary ligation constru ction ofmyocardial ischemia-reperfusion injury model,(1)Too bserve Gualou Xiebai Banxia Decoction improvement of reperf usion injury in rat heart rate disorders;(2) Observed in ra ts serum creatinekinase CK, creatine kinase isoenzyme CK-MBo f change;(3)The use of cardiomyocyteapoptosis by TUNEL assa y, immunohistochemical detection of myocardial Bcl-2, Baxan d P38MAPK protein expression changes;(4)Through an optical microscopeThe observation of myocardial ischemia andreperfu sion injury in rat cardiac morphological changes.Result:(1) The Gualou Xiebai Banxia Decoction can be effect ive against myocardial ischemia reperfusion injury caused b yabnormal heartrhythms, and compared with I/R group, thedif ference was statistically significant (P<0.01、P<0.05);(2) Gualou Xiebai Banxia Decoction can effectively inhibit myoca rdial cell injury caused by reperfusion, reduce CK、CK-MB lea kage,compared with I/R group, the difference was statistica lly significant (P<0.01、P<0.05);(3) Compared with model g roup,Gualou Xiebai Banxia Decoction can effectivelyinhibit t heoccurrence of myocardial reperfusin apoptosis,thereby redu cing reperfusion injury;(4) Gualou Xiebai Banxia Decoction group of Bc1-2protein expression wassignificantly increase d, a significant reduction of Bax protein expression, Compare d with I/R group, the difference was statistically significa nt (P<0.01、P<0.05);(5) The GualouXiebai Banxia Decoction c an improve effectively activity ofP38MAPK, thus inhibiting a poptosis;(6) By cell morphology, Gualou Xiebai Banxia Deco ction can effectively protect the cardiac tissue structure, inhibitionof reperfusion injury.Conclusion:(1) Gualou Xiebai Banxia Decoction with signifi cant improvements in reperfusion injury-induced arrhythmia;(2) Gualou XiebaiBanxia Decoction can effectively improve the organizational structure damage caused by myocardial re perfusion injury and reduce CK, CK-MB leakage protection of the myocardial tissue;(3) The effect mechanism of Gualou X iebai Banxia Decoction can effectively inhibit myocardial i schemia and reperfusion injury induced cardiac myocyteapopt osis may related to regulate Bcl-2/Bax protein expression a ndactivation of P38MAPK.

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