The Efect of Different Intensity Exercise on Diabetes Atherosclers and Serum Levels of NF-kb、IL-6、IL-10and TNF-α
|School||Guangxi Medical University|
|Keywords||diabetes atherosclerosis exercise intensity inflammation factors|
Objective:to evaluate the change of the level of factors NF-KB、IL-6、IL-10and TNF-a in serum of diabetes atherosclerosis rat and the change of arterial vessel wall after different intensity exercise by establishing the model of diabetes atherosclerosis with Sprague-Dawley rats. Indentify the relation between exercise and the arterial vessel wall of diabetes atherosclerosis rat, exercise and inflammation factors NF-KB、IL-6、IL-10and TNF-a, provide the theory of the treatment of diabetes complications by exercise.Methods:(1)100rats were divided into control group(20rats) and diabetes atherosclerosis group(80rats) randomly, the diabetes atherosclerosis group were divided into four subgroups:model group, low intensity exercise group, moderate intensity exercise group, high intensity exercise group,20rats every group. The control group was given normal diet, four subgroups were given high-fat-high-sugar diet (add10%fat and10%sugar and5%egg to normal diet). 4weeks later, the control group was injected with vehicle of0.1mol/1citric acid buffer via abdominal, four subgroups were injected low-dosage of30mg/kg streptozotocin (STZ) to establish rat model of diabetes mellitus. Then continued their diets as before for6weeks until the model of diabetes atherosclerosis were established.(2) Observed the exhaustion time of diabetes atherosclerosis animals in swimming exercise, then the exhaustion time was determined as the time of high intensity exercise,70%and40%of the exhaustion time were determined as the time of moderate intensity exercise and low intensity exercise respectively, the exact time in this study were30min,21min,12min. There different exercise intensity groups swam6d/w, for6weeks. Then all animals were killed by decapitation with anesthesia, the blood and aorta were immediately collected.The levels of factors NF-KB、IL-6、IL-10and TNF-a were measured by ELISA. The arterial vessel wall tissues were fixed with formalin, made paraffin blocks, and seetions of arterial vessel wall were stained with hematoxylin and eosin(HE), then analyzed their pathological changes through the microscope.Results:(1) the rat aorta are serially sectioned, the aortas of control group don’t have any abnormality. aortas of diabetes atherosclerosis group are mainly in the IV phase of atherosclerosis, aortas of low intensity exercise group and high intensity exercise group are mainly in the Ⅱ phase of atherosclerosis, aortas of moderate intensity exercise group are mainly in the I phase of atherosclerosis. (2) Compared to control group, the levels of NF-KB, IL-6, IL-10and TNF-a of four subgroups are all higher than control group either before exercise or after exercise(P<0.001); compared to model group, the levels of NF-KB, IL-6, IL-10of three exercise groups are reduced after exercise (P<0.001); compared to low intensity exercise group, the levels of NF-KB and IL-10of moderate intensity exercise group are lower after exercise (P<0.05), the level of IL-6of moderate intensity exercise group and high intensity exercise group are lower after exercise too(P<0.001); compared to moderate intensity exercise group, the level of IL-10of high intensity exercise group is higher(P<0.001)after exercise; the levels of TNF-a of three exercise groups have no difference with model group after exercise (P>0.05), and there is no difference among three exercise groups either(P>0.05). When every group compared to themselves before exercise, except the control group, the levels of NF-KB, IL-6, IL-10of four subgroups decreased obviously(P<0.001), but not TNF-a.(3) The degree of pathological changes of the aorta tissue of diabetes atherosclerosis rat was positively correlated with the serum levels of NF-KB, IL-6, IL-10and TNF-a, the correlation coefficients were (r=0.773, P<0.05)、(r=0.765, P<0.05)、(r=0.797, P<0.05)、(r=0.532, P<0.05) respectively.Conclusions:exercise plays an important role in the prevention, delay of the formation of diabetes atherosclerosis, especially the moderate intensity exercise. The levels of factors NF-KB, IL-6, IL-10, TNF-α markedly increase in diabetes atherosclerosis. Exercise can reduce the levels of factors NF-KB, IL-6, IL-10, TNF-α of diabetes atherosclerosis rat, but the effect isn’t directly proportional to the intensity of exercise, the moderate intensity exercise have higher effect of factors NF-KB, IL-6, IL-10compared to the other two intensity exercise, the three different intensity exercise have no effect on TNF-α. The degree of pathological changes of diabetes atherosclerosis was positively correlated with the serum levels of NF-KB, IL-6, IL-10and TNF-α.