Dissertation
Dissertation > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes

The Relationship of Glutamine with Type2Diabetes and its Mechanism Research

Author LiuJing
Tutor LiLinLin
School Xinjiang Medical University
Course Pharmacology
Keywords Glutamine type2Diabetes Uygur Kazak Bacteroides thetaiotaomicron
CLC R587.1
Type Master's thesis
Year 2013
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Objective: To study the glutamine(Gln) and the relationship with type2diabetes, and its effect to fasting blood-glucose(FBG)、blood lipids and target bacteria inintestines act on diabetic mice. Methods:①Use HPLC to determine the content ofglutamine in Plasma of Uygur and Kazak ethnic.To determine fasting serum insulin(FINS) by using the radioimmunoassay and TC,TG,HDL-C,LDL-C through automatic biochemical analyzer.②Alloxan-induced diabetes mice, to observe the change ofweight and FBG after giving Gln intervene to model+Gln group and Gln group.The content of C-Peptide and the glutamine content were determined by enzyme-linked immunosorbent assay (ELISA) in the serum of the mice. To determine TC, TG, HDL-C, LDL-C in serum of the mice, by using automatic biochemical analyzer.③DNA was extracted from fecal samples of mice, the level of the bacteroides thetaiotaomicron was determined by Real-time PCR. Result:①The glutamine contentwas significant difference between NGT’s and T2DM in the Uygur(P<0.01), but there was no significant difference between the NGT’s and T2DM in the Kazak;There was significant difference between the Uygur and Kazak normal person (P<0.01), there was significant difference between the Uygur and Kazak, who with type2diabetes mellitus (P<0.05). Glutamine content was negatively correlated with TC(r=-0.4483, P=0.006) in Kazakh people. In Uygur people, glutamine content was positively related with HOMA-IS(r=0.52,P=0.001),but negatively with FBG(r=-0.61,P<0.001), HOMA-IR(r=-0.55,P<0.05),TG(r=-0.5064,P<0.05), LDL-C(r=-0.5621,P=0.008).②The Gln have effect on Gln group and model+Gln group: compared with blankcontrol group, Gln could reduce the weight of Gln group after giving Gln, and there was significant difference(P<0.01), model+Gln group compare with model group,there was significant difference from the4th week(P<0.05); About FBG, there wassignificant difference on week3and4(P<0.05), between model+Gln group and m odel group; About C-peptide compare with model group, model+Gln group and Gln group all have significant difference(P<0.05); There were significant differences(P<0.05)about IRI, compared with model group, in model+Gln group and Gln group; About HOMA-IS, compared with model group, model+Gln group and Gln group all have significant difference(P<0.01). Obtain by correlational analyses, glutamine content was negatively correlated with FBG(r=-0.45,P<0.05), HOMA-IR(r=0.3216,P<0.05), LDL-C(r=-0.5091,P<0.01) and positively correlated with C-peptide(r=0.4584,P<0.05),HOMA-IS(r=0.3216,P<0.05).③Alloxan was able to induce diabetes in35%(7/20) mice for Gln group, but Alloxan was able to induce diabetes in50%(10/20)mice for the control group.④There was not significant change for Colonic pathological section, and model+Gln group compare with model group, there was no significant difference in Bacteroides thetaiotaomicron. Concludes: There was significant difference of the glutamine content between Uygur and Kazak, That may be show glutamine maybe a protective factors to type2diabetes. Gln could reduce theweight and FBG of the diabetic mice, and increase secretion of the C-peptide、HOMA-IS, that may be show, that the Gln could have effective for diabetes. Model+Gln group compare with model group, there was no significant difference in Bacteroides thetaiotaomicron. that may be show, hypoglycemic effect of Gln was not happened by Bacteroides thetaiotaomicron.

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