Dissertation
Dissertation > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes

The Expression of AQP-2in Kidney of Diabetic Rats and the Intervention Effect of Aerobic Chronoexercising

Author GuoJingJie
Tutor WuMingFang
School Suzhou University
Course Human Movement Science
Keywords aerobic Chronoexercising T2DM AVP PKA AQP-2
CLC R587.1
Type Master's thesis
Year 2012
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Objective:To observe the relationship between the expresion of AQP-2in Type2diabetes(T2DM) rat and the changes in plasma indicators, and the effect of aerobicChronoexercising on the expresion of AQP-2and the changes in plasma indicators, theT2DM rat model was induced by high glucose and high fat feedstuff and STZ,theaerobic exercise was intervented bases on the blood glucose fluctuationsin rhythm characteristics.Methods:Randomly selected12from80male rats as healthy control group (H, N=12) andthe rest was diabetic model group (G, N=68), in which the health group wasconventional breeding without exercise, and the diabetes model group was feeded withhigh glucose and high fat feedstuff and Streptozotocin. After the success of modeling,14rate were randomied into diabetic control group (C, N=14) with conventionalbreeding without exercise. The rest of diaretic rats were divided into the6clockexercise group (M, N=14),12clock exercisegroup (N,N=14) and18clock exercisegroup (E, N=14). The blood glucose levels in all rats were determined on prior andafter the modeling. The tissue of kidney were removed after the experiment, bloodsamples were also collected. ELISA method was explored to detect the expression ofFINS and related indicators(IR,IAI Homa-β), AVP, PKA, AQP-2. All analysis wereconducted using SPSS17.0Results:The level of blood glucose in health group was higher in18:00compared with6:00(p <0.01), there were no significant different in the expression of insulin、AVP、PKA and AQP-2between the two groups (p>0.05).The level of blood glucose in T2DM rats was higher than normal (p <0.01), asimilar result in the expression of insulin、AVP、PKA and AQP-2between the twogroups. No different was observed in level of blood glucose between the8:00and6:00.The weight in S group was decreased after aerobic chrono exercising (p <0.01). Inthe subgroup, compared with SN group, the weight in SE group and SM group wassignificantly decreased (p <0.01).The level of blood glucose in S group was lower than C group after aerobicchronoexercising (p <0.01), was also lower than the same time before exercise (p <0.05). In the subgroup, compared with SM group, the level of blood glucose in SEgroup and SN group was significantly decreased (p <0.01).The level of FINS was lower in S group compared with C after aerobicchronoexercising (p <0.01). In the subgroup, there were no significant different.The level of Homa-IR in S group was lower than C group in diabetic rats afteraerobic chronoexercising (p <0.01). Compared with SM group, the level in SN groupand SE group was significant lower (p <0.01). However, the level of blood glucose inSE group and SN group was significantly higher (p <0.01).The expression of AVP and AQP-2was decreased in S group, compared with Cgroup in T2DM rats after aerobic chronoexercising. In the subgroup, the expression ofAVP and AQP-2in SM and SN group was lower than SE group.ConclusionThe level of blood glucose had circadian rhythm in healthy rats. The level of bloodglucose increased in the diabetic rats, and the circadian rhythm was disorder,accompanied with insulin resistance, compensatory increase of renal urineconcentration functionThe intervention of aerobic chronoexercising could improve insulin function, decreasethe level of blood glucose, especially in the peak level, but there was on effect on thecircadian rhythm.The intervention of aerobic chronoexercising could improve the polyuria in diabetic rats, reduce the loss of body water, especially in the peak level

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