Fermentation Condition Optimization, Characteristics and Inhibition on Mango Anthrax of Antibiotic Substance Produced by Trichoderma Viride
|School||Sichuan Agricultural University|
|Course||Agricultural Products Processing and Storage|
|Keywords||Viride Mango anthrax Antibiotics Fermentation Stability Antibacterial spectrum Induced Resistance|
This paper studies the metabolism of Trichoderma green mango anthrax antibiotic in vitro inhibition, as well as damage to anthrax vaccination mango mango anthracnose control effect . The use of liquid fermentation of biomass to obtain anti- metabolites , optimal fermentation conditions by single factor test , Plackett-burman test and response surface CCD experimentally determined as follows: On the basis of the basic culture medium with glucose as carbon source , dosage 20g / L, supplemental nitrogen to corn flour, dosage 10g / L, bottling capacity of 50mL/250mL conical flask (△), fermentation temperature is 33.6 ℃, fermentation seed strains of Trichoderma green age of 5d, surfactants spit temperature -80 dosage 1g / L, initial pH of 10, the fermentation metabolites mango anthrax antibiotic inhibition rate ( 81.47 4.03 ) % , 80.56% and very close to the maximum predicted . In a study of Trichoderma antibiotic fermentation broth stability , and found that it has good thermal stability. When the treatment temperature is 100 ℃, 30min after inhibition of mango processing Anthrax still up 71.51 ± 0.54%. Against acid treatment of biomass has some damaging effects , but the fermentation broth at pH 2 inhibitory activity was 73.23 ± 0.89%, 12% higher than the untreated control group . Na, K, Mg2, Fe2 antibacterial activity under the effect of the difference is not obvious , but Fe3 treated viride fermentation broth can be 100 % inhibition of anthrax mangoes grown on PDA medium . Biomass against oxidant damage has obvious effect ; reducing agent is between antibiotics and there is a certain coordination synergies. In addition to its bovine trypsin have some damaging effects , other enzymes obvious. Viride metabolic antibiotic broad spectrum antimicrobial substance , when the concentration ratio of 8:00 , for S. aureus, B. subtilis, B. cereus and P. vulgaris inhibition zone diameter was 33.6 ± 0.2mm respectively , 33.8 ± 0.9mm, 29.1 ± 0.3mm and 14.9 ± 0.1mm. MIC determination results as : S. aureus and B. cereus the MIC was 2 -fold concentrated broth ; B. subtilis the MIC was 4 -fold concentrated broth ; at the test concentration has not been selected broth MIC values of P. vulgaris . Fusarium oxysporum, C. gloeosporioides, Aspergillus niger and Paecilomyces Bainier half inhibitory concentration (IC50) were 1.37,1.39,4.24 and 4.07 ( concentrated multiply ) . Through statistical fruit number and size of the lesion , indicating viride treatment significantly reduced the damage inoculated mango rot index lesion area and vaccination morbidity. By measuring the quality of fruit each treatment group showed that fruit firmness , color change viride fermentation treatment group , soluble solids and the control group was not significantly different . Slow fermentation process and increase the protein content of the fruit fall around the lesion titratable acid , ascorbic acid increased the (6-8d) content, reducing the malondialdehyde (6-9d) content . Fruit defensive activity assay showed that Trichoderma green broth inoculation injury treatment inhibited fruit PPO (7d) activity increased, decreased SOD activity slowed , inhibited POD (5-7d) activity. In addition, the ability to remove the lesion fruit around DPPH significantly increased compared with the control (5-9d), total phenol content of fruit also increased significantly . CAT activity and FRAP did not change significantly . Isoenzyme test results show that , POD isozymes fewer , but changes in large differences between each treatment ; PPO isozymes large number , but the change is small. Days in the control group compared with the same treatment group PPO isozymes increased. SOD isozyme bands appear later time than the control , the number of bands to be more than over the same period .