The Effect of Moderate-severe Hemodilution on Rabbit Intestine
|Keywords||Hemodilution intestine Occludin AQP-1 Endotoxin|
Objective To Discuss the effect and mechanism of moderate-severehemodilution in different methods on intestine by using multiple study methods.Methods72rabbits were divided into three groups randomly accord tohemodilution method and20%was set as target hematocrit: control or C group （nothemodilution after molding）, ANH or N group （dilute to20%by acute normovolemichemodilution）, AHH or H group （dilute to20%by acute hypervolemic hemodilution）.Four time point were set according to maintenance time after hemodilution: T0（justbefore hemodilution）, T1（1h after hemodilution）, T2（2h after hemodilution）, T3（4hafter hemodilution）. Vena blood and ileum sample were taken at T0, T1, T2, T3in eachgroup. The content of intestine mucosa Occludin and blood endotoxin were measuredby ELISA method. The expression of intestine AQP-1was measured byimmunohistochemistry. The content of water in intestine was measured by dry-wetweight method. Intestine pathology was observed under microscope.Results There was no obvious difference at T0time point in each indexbetween groups （P>0.05）. Each index of diluted groups was compared with relativeone in C group at the same time point. Occludin: there was no statistical difference atT1, T2in N group and T1in H group （P>0.05）, and there was a significant decrease atT3in N group and T2, T3in H group （P<0.01）. AQP-1: there was no statisticaldifference at T1in N group （P>0.05）, and there was a significant decrease at T2, T3inN group and T1, T2, T3in H group （P<0.01）. Blood endotoxin: there was no statisticaldifference at T1, T2, T3in N group and T1, T2in H group （P>0.05）, and there was asignificant increase at T3in H group （P<0.01）. The content of water in intestine: therewas no statistical difference at T1in N group （P>0.05）, and there was a significantincrease at T2, T3in N group and T1, T2, T3in H group （P<0.01）. Pathology: a normalstructure was found at each time point in C group, at T0, T1in N group and T0in Hgroup. There was a mile edema villus at T2in N group. The villus and lamina propria were found edema at T1in H group, with inflammatory cell infiltration. At T3in Ngroup and T2in H group, the edema extended to submucosa, the villus shed,inflammatory cells infiltrated and capillaries hemorrhaged. The mucosa andsubmucosa occured necrosis at T3in H group.Conclusions After the hematocrit is diluted to20%by ANH under generalanesthesia and air-inspired controlled ventilation, the expression of AQP-1in rabbitintestine reduces2h later and the intestine structure shows mild injury, the content ofOccludin reduces4h later and the structure shows moderate injury. For AHH, AQP-1reduces1h later and the structure shows mild injury, Occludin reduces2h later and thestructure shows moderate injury, blood endotoxin increases4h later and the structureshows serious injury. Comparing to ANH, the decrease-occurring time of AQP-1andOccludin in AHH is much earlier, and the intestine injury is more serious at the sametime point. The injury Mechanism of ANH and AHH on intestine may be related withOccludin and AQP-1, and blood endotoxin may reflect severity of intestinal injury.