Dissertation
Dissertation > Medicine, health > Surgery > Of surgery > Abdominal surgery > Pancreas > Pancreatitis

An Experimental Study on the Effect of EGCG in Mice Acute Pancreatitis

Author YangWeiZhen
Tutor PengYanHui; TuoHongFang
School Hebei Medical University
Course Surgery
Keywords EGCG NF-Kb MDA AMS TNF-α IL-6 Acute pancreatitis
CLC R657.51
Type Master's thesis
Year 2012
Downloads 33
Quotes 0
Download Dissertation

Objectives: To study the effect of EGCG on the pancreatic injury inmurine with acute pancreatitis, we could observe the levels of serum AMS,TNF-α, IL-6, MDA, the pathology of pancreas and the activity of NF-κB inpancreas, which could provide theoretical ground for clinical.Methods: Fifty-four bal b/c mice (median weight,23.5g)wererandomly divided into three groups: Control group (group A), acutepancreatitis group (group B) and treatment group with EGCG(group C). Eachgroup was divided into8h、12h、24h subgroups (n=6in each subgroup) afterthe induction of pancreatitis. Acute pancreatitis was induced by serialintraperitoneal administration of cerulein (50μg/kg,7). EGCG (25mg/kg)was administered intraperitoneally at1,3, and6hours after the induction ofpancreatitis;.Mice of group A were only received the same volume of0.9%NaCl without caerulein. Six mice from each subgroup were killed, then thecorrelated indexes were evaluated at8h12h24h after the induction ofpancreatitis.Part of pancreatic tissue was changed by HE staining, then themorphologic changes were evaluated. Immunohistochemical revealedsignificant NF-κB activation of pancreatic tissue.The level of AMS、TNF-α、IL-6、MDA in serum were measured. The data were analyzed with SPSS13.0.Results:1Observation of pancreatic tissue:①Normal pancreatic tissue wasobserved, did not see the edema and necrosis in group A.Edema andhyperemia pancreatic tissue, saponification spot and clear ascites wereobserved in group B. Hyperemia and edema pancreatic tissue was observed,did not see the obvious ascites in group C.②Morphologicobservation: Theclear structure of pancreatic lobules were observed, and interstitialinflammatory cells and red blood cells were not observed in group A. Pancreaslobular disordered arrangement, interlobular distance widened, interlobular visible inflammatory cells and red blood cells were observed in group B.Pancreas interlobular septal thickening wide, inflammatory cell invasionedaround the catheter, interstitial scattered in the distribution of a fewinflammatory cells and red blood cells. in group C.③pancreatichistopathological score results: The scores of pathological changes of pancreasin group B were significantly higher than those of group A at thecorresponding time points (P<0.05). In group B, pathological injury wasgradually aggratated with time was prolonged. There was a significantdifference between group C and group B at the corresponding timepoints(P<0.05).2.The expession of NF-KB in pancreatic tissue in group Bwere significantly higher than those in group A at corresponding timepoints(P<0.05). In group C, The expession of NF-KB in pancreatic tissue weresignificantly lower compared with those in group B at8h12h. The statisticaldifference was significant (P<0.05).3.Compared with group A, serum AMS ingroup B were significantly increased8h,12h,24h after modeling, serum AMSin two groups were significantly different at corresponding time points(P<0.05). Compared with Group B, AMS in serum of Group C wassignificantly reduced8h,12h and24after successful modeling and serumAMS in two groups were significantly different at corresponding time points(P<0.05).4.Compared with group A, serum TNF-a in group B weresignificantly increased8h,12h,24h after modeling, serum TNF-a in two groupswere significantly different at corresponding time points (P<0.05). Comparedwith Group B, TNF-a in serum of Group C was significantly reduced8h,12hand24after successful modeling and serum TNF-a in two groups weresignificantly different at corresponding time points (P<0.05).5. Compared withgroup A, serum IL-6in group B were significantly increased8h,12h,24h aftermodeling, serum IL-6in two groups were significantly different atcorresponding time points (P<0.05). Compared with Group B, IL-6in serumof Group C was significantly reduced8h,12h and24after successful modelingand serum IL-6in two groups were significantly different at correspondingtime points (P<0.05).6. Compared with group A, serum MDA in group B were significantly increased8h,12h,24h after modeling, serum MDA in two groupswere significantly different at corresponding time points (P<0.05). Comparedwith Group B, MDA in serum of Group C was significantly reduced8h,12hand24after successful modeling and serum MDA in two groups weresignificantly different at corresponding time points (P<0.05).7. There was apositive correlation between the levels of MDA in serum and the scores ofpathological changes of pancreas (r=0.754, P<0.05).Conclusion:It is the classic method of the acute Pancreatitis induced bycerulein in mice.Through the experimental study with AP, it confirmed acutepancreatitis can elevate inflammatory factor and oxygen free radical content,further increase the pancreatic tissue injury. The experimental results showthat EGCG can reduce acute pancreatitis mice serum amylase level, has cutpromote inflammation factors(such as NF-KB、TNF-a、IL-6), and enhance thebody of oxygen free radical scavenging capacity,reducing local inflammatorycells infiltrating and pancreas tissue damage effects, EGCG play therapy effecton experimental mice acute pancreatitis.

Related Dissertations
More Dissertations