Dissertation
Dissertation > Medicine, health > Oncology > Respiratory system tumors > Lung tumors

Effects of EGCG Combined with Taxol on the Proliferation and Apoptosis of Human Lung Cancer Cell Line A549

Author WangJiangHong
Tutor GuoShuLiang
School Chongqing Medical University
Course Internal Medicine
Keywords EGCG Taxol Lung Cancer Apoptosis
CLC R734.2
Type Master's thesis
Year 2012
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Objective: To screen To study the effect and possible mechanism ofEGCG combined with taxol on the proliferation and apoptosis of humanlung cancer cell line A549.Methods: The effect on cell viability of EGCG and taxol wereexamined by cell colony formation assay,cell proliferation inhibition wasexamined by WST-1assay,the apoptosis of cells were examined by Heochst33342test, the apoptosis rates were examined by flow cytometry, and theGRP78expression was examined by Western blot.Results: EGCG and taxol either alone or combined inhibited theproliferation and induced apoptosis of human lung cancer cell lineA549.The combination of two drugs was obviously more effective than thatof the drug alone(P<0.05).Conclusions: EGCG and taxol could inhibit proliferation and induceapoptosis of A549cells and up-regulate the expression of GRP78.EGCGand taxol have synergistic effect on lung cancer cell line A549proliferationinhibition.

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