Dissertation > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors

Sirna Silencing Survivin Effect on Nasopharyngeal Carcinoma Cells CNE2

Author HuangXinYi
Tutor ZhengJiaFa
School Nanhua University
Course Department of Otolaryngology Head and Neck Surgery
Keywords SiRNA Survivin nasopharyngeal carcinoma Caspase-3 genesilencing
CLC R739.63
Type Master's thesis
Year 2013
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Objective: Investigate siRNA silence survivin gene expression in nasopharyngealcarcinoma (NPC)cells cycle, value-added, apoptosis and Caspase3, the influence of theenzyme activity, provide new experimental basis for nasopharyngeal carcinoma genetherapy.Method: Design and synthesis of two different specific siRNA interference survivinexpression, by liposome transfection nasopharyngeal carcinoma CNE2cells. Using flowcytometry instrument, detection of cell cycle and cell apoptosis rate; Using CCK8reagentmethod, detection cell value; Using Caspase3kits, detection of Caspase3enzyme activity.Results: After siRNA silences Survivin gene expression, compared with blank controlgroup, negative control group, Survivin siRNA in group a, Survivin siRNA b group ofcells in G0/G1phase, the G1phase of the cell increased, S phase cells reduce (p﹤0.05);Compared with blank control group, Survivin siRNA in group a, Survivin siRNA group bcell proliferation was inhibited (p﹤0.05), Survivin siRNA group b and Survivin siRNAcompared group a, cell proliferation inhibition significantly more (p﹤0.05); Survivinapoptosis rate of siRNA group b (39.5%±0.79) higher than the blank control group(2.65%±0.41), the liposome group (3.25%±0.52), negative control group (4.16%±0.62) and Survivin siRNA in group a (37.2%±0.71)(p﹤0.05); Caspase3activitydetection showed that compared with blank control group, negative control group, SurvivinsiRNA in group a, Survivin siRNA Caspase3enzyme activity of group b (p﹤0.05).Conclusion:1. Survivin gene expression after siRNA silence, inhibits nasopharyngealcarcinoma CNE2cells growth, promoting apoptosis, in G0/G1phase retardation.2. Survivin gene expression after siRNA silence, Caspase-3innasopharyngeal carcinoma CNE2cells enhanced enzyme activity. 3. The different targets of siRNA Survivin gene, impact on nasopharyngealcarcinoma CNE2cells have differences

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