Dissertation > Medicine, health > Ophthalmology > Eye fiber membrane disease > Corneal diseases > Keratitis, corneal ulcers

Experimental Study of MPO MIF iNOs in Keratomycosis with Active Macrophage

Author DongChengZuo
Tutor ZhuXueJun
School Fujian Medical
Course Ophthalmology
Keywords keratomycosis immune response macrophage
CLC R772.21
Type Master's thesis
Year 2012
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Objective To study the role of macrophages in keratomycosis and detect theexpression of relating cotokines in a mouse model of Fusarium solani keratitis.Methods A mouse model of Fusarium solani keratitis was made byepikeratophakia.Macrophage was activated by intracorneal instillation of latex beadsin BALB/c mice with Fusarium solani keratitis, respctively,Controls receivedphosphate-buffered saline(PBS-LIP)instead.The infected corneas were observedthrough slitlamp and histopathological analysis. Cytokine mRNA levels were detectedby real-time polymerase chain reaction.Results In the Latex Beads-treated group,more expression of macrophages could befind in the conjunctiva and cornea,there was a obvious increase in mRNA expressionof relating cotokines.experimental group first,cornea become to be turbidity and inbibition,atday7,cornea infiltrate all full-thickness and some place to be ulcer and perforation controlgroup cornea become to be a little turbidity and inbibition less rate of perforation;HE coloreturexperimental group could find a lot of inflammatory cell,at day7,most of inflammatory cellaggregate;control group coeuld find little of inflammatory cell.Real time-PCR detection:there is MPO MIF iNOs’s mRNAculd be find in experimentalgroup and control group.at day1,the number of MIF MPO iNOs is in control group,in theexperimental group,more and more mRNA to find at day7.(P<0.05)Conclusions Local activation of macrophages may upregulate the immune responseand aggravate fungal keratitis. Macrophages appear to play an importantrole in host defense against corneal infection of F. solani.

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