The Correlation between Mitochondrial DNA Haplogroups and Leprosy in the Han Chinese of Guizhou Province
|School||Zunyi Medical College,|
|Course||Dermatology and Venereology|
|Keywords||Leprosy mitochondrial DNA haplogroup Genetic susceptibility|
Objective:To study the distribution of Mitochondrial DNA haplogroups in different groups Han population of Guizhou province and analysis the correlation between mitochondrial DNA haplogroup and leprosy and investigate the mitochondrial genetic susceptibility to leprosy.Methods:Informed consent was obtained from each patient and the study was approved by relevant ethical review committee,90Han individuals were sampled in Guizhou province, China:30cases of lepromatous leprosy patients,30cases of tuberculoid leprosy patients,30cases of normal controls. The whole-blood of all samples was collected with EDTA anticoagulant, then the mitochondrial DNA was extracted from the whole-blood samples. Hypervariable segment Ⅰ and Ⅱ sequences were amplified by PCR, then the PCR products were sequenced. With the standard sequence comparison to identify mutations, then every sample was roughly divided into haplogroup and subsequently confirmed by screening some specific coding-region sites with the aid of restriction fragment length polymorphism typing and sequencing of some necessary segments. Compare differences in the frequency of mitochondrial DNA haplogroup distribution in the different groups. The analysis of the data was done by using SPSS software version19.0and the pvalue of less than0.05was considered to be significant.Results: There were no significant difference in the distribution frequencies of the mitochondrial DNA haplogroup in the lepromatous leprosy group, tuberculoid type leprosy group and control group (P>0.05).Conclusion:There were no significant differences in the distribution frequencies of the mitochondrial DNA haplogroup in the lepromatous leprosy group, tuberculoid type leprosy group and control group.The mitochondrial DNA haplogroup may be nothing to do with genetic susceptibility to leprosy.